發(fā)布時(shí)間：2018-05-02 16:41

  本文選題：聚乳酸納米粒子 + 免疫佐劑　； 參考：《北京化工大學(xué)》2015年碩士論文

【摘要】：納米技術(shù)是一種很有發(fā)展?jié)摿Φ男滦图夹g(shù)。納米材料,以其粒徑小、比表面積大、性能優(yōu)越等特點(diǎn),具有廣泛的應(yīng)用前景。生物可降解的高分子聚合物制備成的納米粒子在生物醫(yī)藥領(lǐng)域具有研究和應(yīng)用潛力。本論文主要研究聚乳酸納米粒子的制備方法并進(jìn)行工藝優(yōu)化,將制備的聚乳酸納米粒子應(yīng)用于免疫佐劑的研究。取得的具體研究結(jié)果如下：1、研究納米沉淀法制備聚乳酸納米粒子,采用響應(yīng)面優(yōu)化法對(duì)制備工藝進(jìn)行優(yōu)化,最優(yōu)條件制備得到的聚乳酸納米粒子粒徑為109.1 nm,PDI為0.085,粒徑均一,分散性良好；2、研究自乳化-溶劑揮發(fā)法制備聚乳酸納米粒子,對(duì)其進(jìn)行工藝優(yōu)化。采用陰離子表面活性劑十二烷基硫酸鈉(SDS)制備表面帶負(fù)電的聚乳酸納米粒子,優(yōu)化工藝制備的聚乳酸納米粒子的粒徑為126.8 nm, PDI值為0.129, Zeta電位為-53.7 mV,粒徑均一,分散性良好；采用陽(yáng)離子表面活性劑雙十二烷基二甲基溴化銨(DDAB),制備表面帶正電的聚乳酸納米粒子,優(yōu)化工藝制備得到納米粒子的粒徑為101.7 nm, PDI值為0.078,Zeta電位為+37 mV,粒徑均一,分散性良好；3、將帶正電的聚乳酸納米粒子作為免疫佐劑開(kāi)展免疫效果研究,考察了100 nm、300 nm和1μm三種粒徑的聚乳酸顆粒,測(cè)定不同粒徑免疫佐劑效果,結(jié)果表明100 nm聚乳酸納米粒子能誘導(dǎo)更高水平的血清特異性抗體、細(xì)胞因子等表達(dá),免疫效果更好；4、研究了表面帶負(fù)電的聚乳酸納米粒子的溶脹制孔,分別采用二次乳液溶脹法、載氣溶脹法和動(dòng)態(tài)溶脹法三種方法進(jìn)行納米粒子的制孔研究,對(duì)三種方法的實(shí)驗(yàn)裝置、實(shí)驗(yàn)條件進(jìn)行優(yōu)化,成功地在納米粒子內(nèi)部制出孔道。
[Abstract]:Nanotechnology is a new technology with great potential for development. Nanomaterials, with their small particle size, large specific surface area, superior performance and other characteristics, have a wide range of application prospects. The nanoparticles prepared by biodegradable polymer have the potential of research and application in the field of biomedicine. In this paper, the preparation method of polylactic acid nanoparticles was studied and the preparation process was optimized. The prepared polylactic acid nanoparticles were applied to the study of immune adjuvant. The specific results obtained are as follows: 1. The preparation of polylactic acid nanoparticles by nano-precipitation method was studied. The preparation process was optimized by response surface optimization method. The particle size of polylactic acid nanoparticles prepared under the optimum conditions was 0.085 and 0.085, and the particle size was uniform. Poly (lactic acid) nanoparticles were prepared by self-emulsifying-solvent volatilization method. Polylactic acid nanoparticles with negative charge were prepared by anionic surfactant sodium dodecyl sulfate. The particle size, PDI value and Zeta potential were 126.8 nm, 0.129 and -53.7 MV, respectively. The polylactic acid nanoparticles with positive charge on the surface were prepared by using the cationic surfactant didodecyl dimethylammonium bromide (DDABN). The particle size of the nanoparticles was 101.7 nm, the PDI value was 0.078% Zeta potential was 37 MV, and the particle size was uniform. The immunological effect of polylactic acid nanoparticles with positive charge as immune adjuvant was studied. Three kinds of polylactic acid particles (100nm ~ 300nm and 1 渭 m) were investigated. The results showed that 100nm polylactic acid nanoparticles could induce a higher level of serum specific antibodies, cytokines and other expressions, and the immune effect was better. The swelling of polylactic acid nanoparticles with negative charge on the surface was studied. The pore preparation of nanoparticles was studied by means of secondary emulsion swelling method, gas carrying swelling method and dynamic swelling method respectively. The experimental equipment and experimental conditions of the three methods were optimized, and the pore channels were successfully made in the nanoparticles.
【學(xué)位授予單位】：北京化工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：O633.14;TB383.1

【共引文獻(xiàn)】

相關(guān)期刊論文 前7條

1 王勤;王傳棟;劉陽(yáng);馬麗霞;;L-丙交酯/ε-己內(nèi)酯配比對(duì)共聚物微結(jié)構(gòu)和性能的影響[J];高分子材料科學(xué)與工程;2012年06期

2 趙靜娜;;醫(yī)用手術(shù)縫合線的研究應(yīng)用現(xiàn)狀[J];國(guó)外絲綢;2008年02期

3 吳航;張麗芳;熊左春;馬馳;陳棟梁;熊成東;;酯交換反應(yīng)條件對(duì)聚(L-丙交酯-co-ε-己內(nèi)酯)力學(xué)性能的影響[J];合成化學(xué);2011年05期

4 任元元;蘭建武;;可生物降解醫(yī)用縫合線的研究進(jìn)展[J];合成纖維工業(yè);2007年01期

5 徐倩;齊魯;;可吸收手術(shù)縫合線的研究進(jìn)展[J];合成纖維;2007年11期

6 魯越;熊左春;李慶;熊成東;陳棟梁;;聚(L-丙交酯-co-ε-己內(nèi)酯)力學(xué)性能和降解性能研究[J];塑料工業(yè);2010年08期

7 李健;袁曉燕;;合成可吸收縫合線的研究進(jìn)展[J];天津工業(yè)大學(xué)學(xué)報(bào);2006年01期

相關(guān)博士學(xué)位論文 前2條

1 戴曉暉;生物降解脂肪族聚酯與聚準(zhǔn)輪烷及其含糖嵌段共聚物的合成與性能研究[D];上海交通大學(xué);2008年

2 高峰;人造纖維織物外支架—牛頸靜脈帶瓣管道復(fù)合體的制備研究[D];中南大學(xué);2009年

相關(guān)碩士學(xué)位論文 前5條

1 李健;乙交酯-L-丙交酯共聚物纖維拉伸過(guò)程的結(jié)構(gòu)與性能[D];天津大學(xué);2007年

2 劉正華;生物血管用外敷人造纖維編織外支架的組織反應(yīng)性[D];中南大學(xué);2009年

3 沈斌;聚乳酸/蒙脫土納米復(fù)合材料的制備及性能[D];北京化工大學(xué);2010年

4 胡晶;聚己內(nèi)酯/丙交酯聚酯共聚物的合成及性能研究[D];武漢理工大學(xué);2012年

5 袁晨;可降解鎂/聚乳酸復(fù)合材料的制備與性能研究[D];遼寧大學(xué);2013年

，

本文編號(hào)：1834688