基于納米材料的毛細(xì)管電泳分離檢測性能研究及應(yīng)用
發(fā)布時間：2018-08-04 13:54
【摘要】：本論文研究了基于納米材料的毛細(xì)管電泳分離檢測性能研究以及其在生物樣品分析中的應(yīng)用。首先,研究了量子點(diǎn)的合成及機(jī)理,成功合成了CdSe和巰基乙酸(TGA)包被的CdSe/CdS量子點(diǎn),并對兩種量子點(diǎn)從尺寸上和發(fā)光性質(zhì)方面進(jìn)行了詳細(xì)的表征和各種光譜掃描測定。將毛細(xì)管電泳與量子點(diǎn)電化學(xué)發(fā)光分析相結(jié)合,研究了量子點(diǎn)的電化學(xué)發(fā)光性能。在此基礎(chǔ)上,研究了生物胺分子對量子點(diǎn)電化學(xué)發(fā)光的增強(qiáng)作用,構(gòu)建了毛細(xì)管電泳量子點(diǎn)電化學(xué)發(fā)光分析平臺檢測生物胺,研究了生物胺催化量子點(diǎn)電化學(xué)發(fā)光的反應(yīng)機(jī)理。本文還研究了毛細(xì)管電泳-酶聯(lián)免疫分析法檢測海洋魚類樣品中CTXIB毒素。通過制作HRP和Ab標(biāo)記的金納米粒子作為探針,利用金納米粒子的放大作用和毛細(xì)管電泳電化學(xué)檢測相結(jié)合檢測分析了魚類樣品中一種擴(kuò)增免疫毒素CTXIB。全文共分為四章：第一章,詳細(xì)介紹了毛細(xì)管電泳的基本理論,系統(tǒng)概括了毛細(xì)管電泳的基本原理、儀器操作、進(jìn)樣方法的選擇等；從不同的角度、各個方面介紹了毛細(xì)管電泳的研究現(xiàn)狀及應(yīng)用前景。介紹了量子點(diǎn)的概念以及特點(diǎn)、量子點(diǎn)的制備方法和應(yīng)用前景,重點(diǎn)介紹了量子點(diǎn)的電化學(xué)發(fā)光原理和發(fā)光方式。對納米材料的概念及特點(diǎn)作了簡要介紹,重點(diǎn)對納米金及其制備方法作了詳細(xì)的概述,并介紹了納米金的應(yīng)用。第二章,制備了水溶性量子點(diǎn)CdSe和CdSe/CdS合金量子點(diǎn),利用電鏡和光譜儀器對制備的量子點(diǎn)從尺寸上以及發(fā)光性質(zhì)方面進(jìn)行了表征。探究了影響量子點(diǎn)合成的各種因素以及量子點(diǎn)的合成機(jī)理及其結(jié)構(gòu)組成。第三章,探究了基于納米材料的毛細(xì)管電泳量子點(diǎn)電化學(xué)發(fā)光檢測生物胺的研究。包括電化學(xué)檢測平臺的建立,對實(shí)驗(yàn)體系中的緩沖溶液的種類、緩沖溶液的pH值、緩沖溶液的濃度、毛細(xì)管兩端所加的分離電壓、所設(shè)置的檢測電位、毛細(xì)管的進(jìn)樣時間、電位掃描速率等因素在內(nèi)的諸多條件進(jìn)行了詳細(xì)優(yōu)化,確定了最佳檢測條件。并在最佳檢測條件下,對6種生物胺樣品進(jìn)行了分離檢測,不同生物胺分子對量子點(diǎn)電化學(xué)發(fā)光信號有不同程度的增強(qiáng)作用。第四章,利用納米金粒子的放大作用通過毛細(xì)管電泳和電化學(xué)檢測相結(jié)合分離檢測了魚類樣品中一種擴(kuò)增免疫毒素CTXIB的研究。介紹了納米金的合成、用辣根過氧化酶和抗體(Ab)修飾納米金粒子,獲得了辣根過氧化酶和抗體標(biāo)記的納米金探針,納米金可以大量吸附游離的HRP和Ab從而使檢測的靈敏度大大加強(qiáng)。采用非競爭性免疫分析反應(yīng)模式,利用電化學(xué)檢測方法檢測了魚類樣品中免疫毒素CTXIB。結(jié)論：對前文中所述實(shí)驗(yàn)進(jìn)行了簡單的總結(jié)概括。
[Abstract]:In this paper, the performance of capillary electrophoresis based on nanomaterials and its application in biological sample analysis were studied. Firstly, the synthesis and mechanism of quantum dots were studied. CdSe/CdS quantum dots coated with CdSe and (TGA) were successfully synthesized. The size and luminescence properties of the two quantum dots were characterized in detail and various spectral scanning measurements were carried out. The electrochemical luminescence properties of quantum dots were studied by combining capillary electrophoresis with electrochemical luminescence analysis of quantum dots. On this basis, the enhancement effect of biogenic amine molecules on electrochemiluminescence of quantum dots was studied. A capillary electrophoresis platform was constructed for the detection of biogenic amines, and the reaction mechanism of biogenic amines catalyzed by biogenic amines was studied. Capillary electrophoresis-enzyme-linked immunosorbent assay (Elisa) for the detection of CTXIB toxin in marine fish samples was also studied. By making gold nanoparticles labeled with HRP and Ab as probes, the amplification of gold nanoparticles and the electrochemical detection of capillary electrophoresis were used to detect and analyze a kind of amplified immunotoxin CTX B in fish samples. The paper is divided into four chapters: the first chapter introduces the basic theory of capillary electrophoresis in detail, systematically summarizes the basic principle of capillary electrophoresis, instrument operation, selection of sampling methods, etc. The present situation and application prospect of capillary electrophoresis were introduced. In this paper, the concept and characteristics of quantum dots, the preparation methods and application prospect of quantum dots are introduced. The electrochemiluminescence principle and luminescence mode of quantum dots are emphatically introduced. The concept and characteristics of nanomaterials are briefly introduced, and the application of nanocrystalline gold is introduced in detail. In chapter 2, water-soluble quantum dots (CdSe) and CdSe/CdS alloy quantum dots (QDs) were prepared. The size and luminescence properties of the QDs were characterized by electron microscopy and spectrometer. The factors affecting the synthesis of quantum dots, the synthesis mechanism and structure composition of quantum dots are discussed. In chapter 3, the electrochemiluminescence (ECL) detection of biogenic amines based on nanomaterials by capillary electrophoresis quantum dots (QDs) was investigated. Including the establishment of electrochemical detection platform, the type of buffer solution in the experimental system, the pH value of buffer solution, the concentration of buffer solution, the separation voltage added at both ends of the capillary, the detection potential set up, the injection time of the capillary, Many factors, such as potential scanning rate, were optimized in detail and the best detection conditions were determined. Six biogenic amines were separated and detected under the best detection conditions. Different biogenic amine molecules enhanced the electrochemiluminescence signal of quantum dots in different degrees. In chapter 4, the amplification effect of gold nanoparticles was used to separate and detect an amplified immunotoxin CTXIB in fish samples by capillary electrophoresis and electrochemical detection. The synthesis of gold nanoparticles was introduced. The gold nanoparticles were modified with horseradish peroxidase and antibody (Ab), and the nano-gold probes labeled with horseradish peroxidase and antibody were obtained. Nanocrystalline gold can adsorb free HRP and Ab in large quantities, so that the sensitivity of detection can be greatly enhanced. The immunotoxin CTXB in fish samples was detected by electrochemical assay using a non-competitive immunoassay model. Conclusion: the experiments mentioned above are summarized briefly.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TB383.1;O658.9
本文編號：2164093
[Abstract]:In this paper, the performance of capillary electrophoresis based on nanomaterials and its application in biological sample analysis were studied. Firstly, the synthesis and mechanism of quantum dots were studied. CdSe/CdS quantum dots coated with CdSe and (TGA) were successfully synthesized. The size and luminescence properties of the two quantum dots were characterized in detail and various spectral scanning measurements were carried out. The electrochemical luminescence properties of quantum dots were studied by combining capillary electrophoresis with electrochemical luminescence analysis of quantum dots. On this basis, the enhancement effect of biogenic amine molecules on electrochemiluminescence of quantum dots was studied. A capillary electrophoresis platform was constructed for the detection of biogenic amines, and the reaction mechanism of biogenic amines catalyzed by biogenic amines was studied. Capillary electrophoresis-enzyme-linked immunosorbent assay (Elisa) for the detection of CTXIB toxin in marine fish samples was also studied. By making gold nanoparticles labeled with HRP and Ab as probes, the amplification of gold nanoparticles and the electrochemical detection of capillary electrophoresis were used to detect and analyze a kind of amplified immunotoxin CTX B in fish samples. The paper is divided into four chapters: the first chapter introduces the basic theory of capillary electrophoresis in detail, systematically summarizes the basic principle of capillary electrophoresis, instrument operation, selection of sampling methods, etc. The present situation and application prospect of capillary electrophoresis were introduced. In this paper, the concept and characteristics of quantum dots, the preparation methods and application prospect of quantum dots are introduced. The electrochemiluminescence principle and luminescence mode of quantum dots are emphatically introduced. The concept and characteristics of nanomaterials are briefly introduced, and the application of nanocrystalline gold is introduced in detail. In chapter 2, water-soluble quantum dots (CdSe) and CdSe/CdS alloy quantum dots (QDs) were prepared. The size and luminescence properties of the QDs were characterized by electron microscopy and spectrometer. The factors affecting the synthesis of quantum dots, the synthesis mechanism and structure composition of quantum dots are discussed. In chapter 3, the electrochemiluminescence (ECL) detection of biogenic amines based on nanomaterials by capillary electrophoresis quantum dots (QDs) was investigated. Including the establishment of electrochemical detection platform, the type of buffer solution in the experimental system, the pH value of buffer solution, the concentration of buffer solution, the separation voltage added at both ends of the capillary, the detection potential set up, the injection time of the capillary, Many factors, such as potential scanning rate, were optimized in detail and the best detection conditions were determined. Six biogenic amines were separated and detected under the best detection conditions. Different biogenic amine molecules enhanced the electrochemiluminescence signal of quantum dots in different degrees. In chapter 4, the amplification effect of gold nanoparticles was used to separate and detect an amplified immunotoxin CTXIB in fish samples by capillary electrophoresis and electrochemical detection. The synthesis of gold nanoparticles was introduced. The gold nanoparticles were modified with horseradish peroxidase and antibody (Ab), and the nano-gold probes labeled with horseradish peroxidase and antibody were obtained. Nanocrystalline gold can adsorb free HRP and Ab in large quantities, so that the sensitivity of detection can be greatly enhanced. The immunotoxin CTXB in fish samples was detected by electrochemical assay using a non-competitive immunoassay model. Conclusion: the experiments mentioned above are summarized briefly.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TB383.1;O658.9
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 謝天堯,鄭一寧,莫金垣,賴蓉;一種簡易型毛細(xì)管電泳電導(dǎo)檢測裝置及其應(yīng)用[J];分析測試學(xué)報;2000年03期
，本文編號：2164093
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