含金屬卟啉的水溶性共軛聚電解質(zhì)的設(shè)計、合成及其生物應(yīng)用
發(fā)布時間：2018-09-13 07:28
【摘要】：水溶性共軛聚合物(WSCPs)通常由控制其主要光學(xué)性質(zhì)的π共軛主鏈和控制其水溶性的側(cè)鏈組成。WSCPs在生物醫(yī)學(xué)方面具有很多優(yōu)勢,如高的亮度、大的消光系數(shù)、優(yōu)異的耐光性、低的細(xì)胞毒性和良好的水溶液穩(wěn)定性。金屬卟啉是生命存在的基石之一,在自然界中有獨特的生物學(xué)功能。它們都被廣泛的應(yīng)用在生物傳感,熒光成像,醫(yī)學(xué)和光動力治療等領(lǐng)域。本文結(jié)合WSCPs和金屬卟啉兩者的優(yōu)勢,合成了一類含金屬卟啉的WSCPs材料,研究了其在細(xì)胞成像、磁共振成像(MRI)和光動力治療(PDT)方面的應(yīng)用。1、通過將具有T1加權(quán)成像功能的錳卟啉引入到聚芴基共軛聚合物中,合成了具有熒光/MRI雙模式成像功能的水溶性共軛聚電解質(zhì)。雙親性的化學(xué)結(jié)構(gòu)使其可以在PBS緩沖溶液中經(jīng)過自組裝形成100 nm左右粒徑均一的球形納米粒子,其光物理性質(zhì)經(jīng)過紫外-可見吸收光譜和熒光光譜進(jìn)行了表征,形貌通過射電子掃描顯微鏡(TEM)和動態(tài)光散射(DLS)進(jìn)行了表征。結(jié)果表明水溶性共軛聚合物納米粒子具有強的吸收、高的熒光量子產(chǎn)率、好的穩(wěn)定性和生物相容性、優(yōu)異的細(xì)胞成像和高的弛豫效率值(r1=13.15 mM-1S-1),這些結(jié)果表明其在活細(xì)胞成像和MRI方面有很好的應(yīng)用前景。2、設(shè)計合成了一種含有金屬鈀卟啉的新型共軛聚合物,借助其兩親性的分子結(jié)構(gòu),在水溶液中經(jīng)過自組裝形成30 nm左右的納米粒子。利用核磁(NMR)和凝膠滲透色譜(GPC)對中間體和目標(biāo)聚合物的結(jié)構(gòu)進(jìn)行了表征。利用TEM和DLS對水溶性共軛聚合物納米粒子的形貌進(jìn)行了研究。實驗結(jié)果表明該聚合物納米粒子粒徑均一,具有良好的分散性和穩(wěn)定性,可以進(jìn)行細(xì)胞染色。重要的是,水溶性共軛聚合物納米粒子在光照條件下可以有效地產(chǎn)生單線態(tài)氧,使腫瘤細(xì)胞凋亡,實現(xiàn)了細(xì)胞成像和PDT的目的。
[Abstract]:The water-soluble conjugated polymer (WSCPs) is usually composed of 蟺 conjugated main chains which control its main optical properties and side chains that control its water solubility. WSCPs have many advantages in biomedical fields, such as high brightness, large extinction coefficient, excellent light resistance. Low cytotoxicity and good stability of aqueous solution. Metalloporphyrin is one of the cornerstones of life and has unique biological functions in nature. They are widely used in biosensor, fluorescence imaging, medicine and photodynamic therapy. Combined with the advantages of WSCPs and metalloporphyrin, a class of WSCPs materials containing metalloporphyrin were synthesized, and their cellular imaging was studied. The application of magnetic resonance imaging (MRI) and photodynamic therapy (PDT). 1. By introducing manganese porphyrin with T1 weighted imaging into polyfluorene conjugated polymer, a water-soluble conjugated polyelectrolyte with fluorescence / MRI dual-mode imaging was synthesized. The amphiphilic chemical structure made it possible to self-assemble in PBS buffer solution to form spherical nanoparticles with about 100 nm homogeneous diameter. Their photophysical properties were characterized by UV-Vis absorption and fluorescence spectra. The morphology was characterized by (TEM) and (DLS). The results show that the water-soluble conjugated polymer nanoparticles have strong absorption, high fluorescence quantum yield, good stability and biocompatibility. Excellent cell imaging and high relaxation efficiency (R1C 13.15 mM-1S-1). These results show that it has a good application prospect in living cell imaging and MRI. A novel conjugated polymer containing PD porphyrin was designed and synthesized. With the help of its amphiphilic molecular structure, nanoparticles were self-assembled in aqueous solution for about 30 nm. The structures of intermediates and target polymers were characterized by NMR (NMR) and gel permeation chromatography (GPC). The morphology of water-soluble conjugated polymer nanoparticles was studied by TEM and DLS. The results show that the polymer nanoparticles have uniform particle size, good dispersion and stability, and can be stained by cells. It is important that the water-soluble conjugated polymer nanoparticles can effectively produce singlet oxygen under illumination, which can make tumor cells apoptosis and achieve the purpose of cell imaging and PDT.
【學(xué)位授予單位】：南京郵電大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O631
本文編號：2240477
[Abstract]:The water-soluble conjugated polymer (WSCPs) is usually composed of 蟺 conjugated main chains which control its main optical properties and side chains that control its water solubility. WSCPs have many advantages in biomedical fields, such as high brightness, large extinction coefficient, excellent light resistance. Low cytotoxicity and good stability of aqueous solution. Metalloporphyrin is one of the cornerstones of life and has unique biological functions in nature. They are widely used in biosensor, fluorescence imaging, medicine and photodynamic therapy. Combined with the advantages of WSCPs and metalloporphyrin, a class of WSCPs materials containing metalloporphyrin were synthesized, and their cellular imaging was studied. The application of magnetic resonance imaging (MRI) and photodynamic therapy (PDT). 1. By introducing manganese porphyrin with T1 weighted imaging into polyfluorene conjugated polymer, a water-soluble conjugated polyelectrolyte with fluorescence / MRI dual-mode imaging was synthesized. The amphiphilic chemical structure made it possible to self-assemble in PBS buffer solution to form spherical nanoparticles with about 100 nm homogeneous diameter. Their photophysical properties were characterized by UV-Vis absorption and fluorescence spectra. The morphology was characterized by (TEM) and (DLS). The results show that the water-soluble conjugated polymer nanoparticles have strong absorption, high fluorescence quantum yield, good stability and biocompatibility. Excellent cell imaging and high relaxation efficiency (R1C 13.15 mM-1S-1). These results show that it has a good application prospect in living cell imaging and MRI. A novel conjugated polymer containing PD porphyrin was designed and synthesized. With the help of its amphiphilic molecular structure, nanoparticles were self-assembled in aqueous solution for about 30 nm. The structures of intermediates and target polymers were characterized by NMR (NMR) and gel permeation chromatography (GPC). The morphology of water-soluble conjugated polymer nanoparticles was studied by TEM and DLS. The results show that the polymer nanoparticles have uniform particle size, good dispersion and stability, and can be stained by cells. It is important that the water-soluble conjugated polymer nanoparticles can effectively produce singlet oxygen under illumination, which can make tumor cells apoptosis and achieve the purpose of cell imaging and PDT.
【學(xué)位授予單位】：南京郵電大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O631
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 ;Kumada chain-growth polycondensation as a universal method for synthesis of well-defined conjugated polymers[J];Science China(Chemistry);2010年08期
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