發(fā)布時間：2019-02-16 04:35

【摘要】：生理條件下光學(xué)性質(zhì)穩(wěn)定的雙光子熒光染料在生物成像領(lǐng)域具有廣闊的應(yīng)用前景。我們使用2,4-二甲基-6-羥基嘧啶與4-(N,N-二甲氨基)苯甲醛進(jìn)行縮合反應(yīng),設(shè)計合成了具有雙光子熒光性質(zhì)的化合物2-[(1E)-2-[4-(二甲氨基)苯基]乙烯基]-6-甲基-4(3H)-嘧啶(NHP)。通過質(zhì)譜(MS)、核磁共振波譜(NMR)、紫外可見吸收光譜和熒光發(fā)射光譜等技術(shù)手段表征了其結(jié)構(gòu),研究了其光物理性質(zhì),以及外部環(huán)境改變對其發(fā)射光譜的影響。結(jié)果表明,化合物NHP的最佳吸收峰位于400 nm,最佳發(fā)射峰位于540 nm左右,且熒光發(fā)射不受金屬離子、氨基酸和pH等環(huán)境因素的影響。生物實驗結(jié)果表明,化合物NHP細(xì)胞毒性較小,且具有很好的活細(xì)胞和果蠅腦組織成像效果,是一種較為理想的雙光子熒光生物成像染料。
[Abstract]:Two-photon fluorescent dyes with stable optical properties under physiological conditions have broad application prospects in the field of biological imaging. We used 2o 4-dimethyl-6-hydroxypyrimidine to react with 4- (N- (N-dimethylamino) benzaldehyde, A two-photon fluorescence compound 2- [(1E) -2- [4- (dimethylaminophenyl) vinyl] -6-methyl-4 (3H) pyrimidine (NHP). Was designed and synthesized. The structure was characterized by (MS), NMR spectra, (NMR), UV-Vis absorption spectra and fluorescence emission spectra. The photophysical properties and the effects of the external environment on the emission spectra were studied. The results show that the optimum absorption peak of compound NHP is about 540 nm, and the fluorescence emission is not affected by environmental factors such as metal ions, amino acids and pH. The results of biological experiments show that the compound NHP has less cytotoxicity and has good imaging effect of living cells and Drosophila brain tissue, so it is an ideal two-photon fluorescence biometric dye.
【作者單位】： 南京工業(yè)大學(xué)柔性電子重點實驗室&先進(jìn)材料研究院江蘇先進(jìn)生物與化學(xué)制造協(xié)同創(chuàng)新中心;
【基金】：國家自然科學(xué)基金(81672508,61505076) 江蘇省高校自然科學(xué)研究重大項目(16KJA180004)資助~~
【分類號】：O657.3
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本文編號：2424050