發(fā)布時(shí)間：2019-02-16 15:32

【摘要】：目的研究并建立不同產(chǎn)地丹參莖葉UPLC指紋圖譜,采用電噴霧離子源-四極桿-飛行時(shí)間質(zhì)譜(ESI-QTOF/MS)對其特征峰進(jìn)行定性研究,為丹參地上莖葉資源的綜合開發(fā)利用提供科學(xué)依據(jù)。方法采用UPLC法構(gòu)建丹參莖葉的指紋圖譜,色譜條件為ACQUITY UPLC BEH C18(100 mm×2.1 mm,1.7μm)色譜柱,以乙腈-0.1%甲酸水為流動(dòng)相梯度洗脫,體積流量0.4 m L/min,檢測波長280 nm,柱溫35℃。質(zhì)譜檢測采用負(fù)離子模式;電壓3.0 k V;離子源溫度120℃;霧化溫度400℃;霧化氣800 L/h。同時(shí)應(yīng)用相似度評價(jià)、聚類分析和主成分分析(PCA)對實(shí)驗(yàn)數(shù)據(jù)進(jìn)行處理,以分析5個(gè)不同產(chǎn)地12批丹參莖葉樣品間的相似性及差異性,并通過UPLC/Q-TOF/MS對丹參莖葉共有峰進(jìn)行了定性分析。結(jié)果獲得丹參莖葉UPLC特征圖譜,共有峰13個(gè)。12批丹參莖葉的相似度為0.823~0.997,聚類分析、PCA結(jié)果與相似度分析結(jié)果一致。結(jié)論丹參莖葉UPLC指紋圖譜的構(gòu)建和化學(xué)模式的識別為其質(zhì)量控制和丹參非藥用部位利用價(jià)值的挖掘提供科學(xué)依據(jù)。
[Abstract]:Objective to study and establish the UPLC fingerprint of stems and leaves of Salvia miltiorrhiza from different habitats, and to qualitatively study its characteristic peak by electrospray ion source-quadrupole time-of-flight mass spectrometry (ESI-QTOF/MS). It provides scientific basis for the comprehensive exploitation and utilization of stem and leaf resources on the ground of Salvia miltiorrhiza. Methods the fingerprint of Salvia miltiorrhiza was constructed by UPLC. The chromatographic conditions were as follows: ACQUITY UPLC BEH C18 (100 mm 脳 2.1 mm,1.7 渭 m) column, acetonitrile-0.1% formic acid water as mobile phase gradient elution, volume flow rate 0.4 mL / min. The detection wavelength was 280 nm, and the column temperature was 35 鈩，

本文編號：2424581