a国产,中文字幕久久波多野结衣AV,欧美粗大猛烈老熟妇,女人av天堂

水稻和玉米葉肉細胞以及維管束鞘細胞轉(zhuǎn)錄組分析

發(fā)布時間:2024-06-10 22:47
  C4植物的光合作用效率高于C3途徑的植物接近50%。為了將C4途徑引入C3植物中,需要對調(diào)控光合作用的關(guān)鍵基因的分子作用機理進行研究,特別是了解這些基因葉肉細胞(Mc)和維管束鞘細胞(BSc)中差異表達。因此,在本實驗中的研究目的是:⑴從水稻和玉米中獲得均一Mc和BSc,以便通過利用LCM技術(shù)進行細胞特定RNA測序;⑵分析水稻作為C3植物的特定Mc和BSc基因的差異表達;⑶分析玉米作為C4植物的特定Mc和BSc基因的差異表達。水稻中已經(jīng)鑒定了包括Mc和BSc總共35956個基因,其中BSc特有2068個,Mc特有5842個。參與光合作用的基因有:在Mc中特異性表達的OsSET13與OsSET26(Rubisco LS甲基轉(zhuǎn)移酶底物結(jié)合域)、CAB2R/OsLhcb1.2與RCABP89/OsLhcb2(葉綠素A-B結(jié)合蛋白)、PsaH與PsbX(光系統(tǒng)組分)、未知功能CHP01589、K09122、OsPM8ATP基因,但在BSc中這些基因也往往存在微量表...

【文章頁數(shù)】:86 頁

【學位級別】:博士

【文章目錄】:
摘要
abstract
Chapter1 INTRODUCTION
    1.1 Photosynthetic pathways
        1.1.1 C3 pathway
        1.1.2 C4 pathway
    1.2 Evolution of C4 photosynthetic pathway
        1.2.1 Phylogenetic distribution
        1.2.2 Kranz anatomy
        1.2.3 Molecular regulation of C4 pathways
    1.3 The C4 Rice Project
        1.3.1 Rice
        1.3.2 The C4 Rice Project – Overview
        1.3.3 Previous studies
    1.4 Laser capture microdissection technique
        1.4.1 LCM systems
        1.4.2 LCM applications on plants
    1.5 Aims
Chapter2 MATERIALS AND METHODS
    2.1 Plant materials
        2.1.1 Oryza sativa
        2.1.2 Zea mays
    2.2 LCM sample preparation
        2.2.1 Leaf sample collection and fixation
        2.2.2.Microwave processing
        2.2.3 Microtome sectioning
        2.2.4 LCM
    2.3 RNA Extraction and qualification
        2.3.1 RNA extraction
        2.3.2 RNA integrity testing
    2.4 RNA amplification and RNA-seq
        2.4.1 RNA amplification
        2.4.2 RNA-seq
Chapter3 Mc- and BSc-specific RNA of rice and maize
    3.1 Introduction
    3.2 Results and discussion
        3.2.1 Leaf collection
        3.2.2 Laser capture microdissection
        3.2.3 RNA Extraction and RNA Integrity Testing
    3.3 Discussion
Chapter4 Differential expression of rice Mc and BSc transcriptomes
    4.1 Introduction
    4.2 Results
        4.2.1 RNA-seq and alignment against the rice reference genome
        4.2.2 Differential expression of genes between rice BSc and Mc
    4.3 Discussion
        4.3.1 RNA-seq and alignment against the reference genome
        4.3.2. Differential expression of genes between BSc and Mc
Chapter5 Differential expression of maize Mc and BSc transcriptomes
    5.1 Introduction
    5.2 Results
        5.2.1 RNA-seq and alignment against the reference genome
        5.2.2 Differential expression of genes between BSc and Mc
    5.3 Discussion
        5.3.1 RNA-seq and alignment against the reference genome
        5.3.2 Differential expression of genes between BSc and Mc
CONCLUSION
References
致謝Acknowledgement
作者簡歷RESUME



本文編號:3992014

資料下載
論文發(fā)表

本文鏈接:http://www.wukwdryxk.cn/nykjlw/nzwlw/3992014.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶6ff96***提供,本站僅收錄摘要或目錄,作者需要刪除請E-mail郵箱bigeng88@qq.com
狠狠躁夜夜躁人爽碰88a| 天堂在线| 精品午夜福利在线观看| 丰满的年轻搜子在线观看| 无码精品人妻一区二区三区影院| 国产激情免费视频在线观看| 被闺蜜的男人CAO翻了求饶| 亚洲 丝袜 另类 校园 欧美| av天堂色| zzzo女人另类性生交| 久久综合亚洲色HEZYO国产| 亚洲精品美女久久久久99| 亚洲国产精品久久网午夜| 国产精品1区2区3区在线观看| 粗大挺进尤物人妻中文字幕| 中文字幕大香视频蕉无码| 亚洲精品中文字幕乱码三区| 北川| 精品人妻一区| 成人天堂资源www在线| 第一章少妇初尝云雨69章风 | 欧美成人精品三级网站| freehd18xxxx人妻| 久草大香蕉| 91国内精品白嫩初高生| 国产第一页浮力影院入口| 狼友AV永久网站| 国产福利一区二区精品秒拍| 亚洲一线二线三线AV无码| 在线观看欧美一区二区三区| 夜夜高潮夜夜爽夜夜爱爱| 97色伦综合在线欧美视频| 太仆寺旗| 欧美和黑人XXXX猛交视频| 精品国产91乱码一区二区三区 | 91九色蝌蚪在线| 亚洲性猛交xxxx美女写真| 成人国产免费| 亚洲中文字幕无码中文| 久久婷婷五月综合色精品| 亚洲色精品三区二区一区|