a国产,中文字幕久久波多野结衣AV,欧美粗大猛烈老熟妇,女人av天堂

當(dāng)前位置:主頁(yè) > 醫(yī)學(xué)論文 > 泌尿論文 >

過(guò)表達(dá)前列腺特異性膜抗原和熒光素酶的PC3穩(wěn)定細(xì)胞系的建立及成瘤性鑒定

發(fā)布時(shí)間:2018-09-05 13:20
【摘要】:目的利用慢病毒感染的方法建立能穩(wěn)定表達(dá)前列腺特異性膜抗原(PSMA)和熒光素酶的PSMA~+-PC3細(xì)胞系,并將其接種到裸鼠皮下檢測(cè)其成瘤能力。方法包裝表達(dá)PSMA的慢病毒和表達(dá)熒光素酶的慢病毒,將其感染PC3細(xì)胞后,用嘌呤霉素進(jìn)行篩選,建立能夠穩(wěn)定表達(dá)PSMA和熒光素酶的PSMA~+ -PC3細(xì)胞和僅表達(dá)熒光素酶的PSMA--PC3細(xì)胞;利用流式細(xì)胞術(shù)檢測(cè)PSMA的表達(dá)情況。將PSMA~+ -PC3和PSMA~- -PC3細(xì)胞接種于裸鼠皮下建立荷瘤模型,利用生物發(fā)光成像法觀察其在體內(nèi)的成瘤情況,利用免疫組織化學(xué)染色法檢測(cè)PSMA~+ -PC3和PSMA~- -PC3腫瘤組織中PSMA的表達(dá)情況。結(jié)果成功建立了能夠穩(wěn)定表達(dá)PSMA和熒光素酶的PSMA~+ -PC3細(xì)胞和僅表達(dá)熒光素酶的PSMA~- -PC3細(xì)胞,流式細(xì)胞術(shù)檢測(cè)證實(shí)PSMA~+ -PC3細(xì)胞上PSMA呈陽(yáng)性表達(dá),小動(dòng)物活體成像結(jié)果顯示PSMA~+ -PC3和PSMA~- -PC3細(xì)胞均可有效成瘤,免疫組織化學(xué)染色檢測(cè)證實(shí)PSMA~+ -PC3腫瘤上PSMA的表達(dá)為陽(yáng)性。結(jié)論成功建立了能穩(wěn)定表達(dá)PSMA和熒光素酶的PSMA~+ -PC3細(xì)胞系,并在裸鼠體內(nèi)證實(shí)其可有效成瘤,為靶向PSMA的研究提供了有用的細(xì)胞和動(dòng)物模型。
[Abstract]:Objective to establish a PSMA~ PC3 cell line which can stably express prostate specific membrane antigen (PSMA) and luciferase by lentivirus infection, and then inoculate it into nude mice for subcutaneous detection of its tumorigenesis ability. Methods Lentivirus expressing PSMA and lentivirus expressing luciferase were packaged. After infected with PC3 cells, the cells were screened by purine mycin to establish PSMA~ PC3 cells and PSMA--PC3 cells expressing luciferase only and stable expression of PSMA and luciferase. The expression of PSMA was detected by flow cytometry. PSMA~ PC3 and PSMA~- PC3 cells were inoculated into nude mice subcutaneously to establish tumor-bearing model. The tumor formation in vivo was observed by bioluminescent imaging, and the expression of PSMA in PSMA~ PC3 and PSMA~- PC3 tumor tissues was detected by immunohistochemical staining. Results PSMA~ PC3 cells expressing PSMA and luciferase stably and PSMA~- PC3 cells expressing luciferase only were successfully established. The positive expression of PSMA on PSMA~ PC3 cells was confirmed by flow cytometry. In vivo imaging of small animals showed that PSMA~-PC3 and PSMA~- PC3 cells were all effective in tumorigenesis. The expression of PSMA on PSMA~-PC3 tumor was confirmed by immunohistochemical staining. Conclusion PSMA~-PC3 cell line which can stably express PSMA and luciferase was successfully established and proved to be effective in tumorigenesis in nude mice. It provides a useful cell and animal model for the study of targeted PSMA.
【作者單位】: 第四軍醫(yī)大學(xué)免疫學(xué)教研室;第四軍醫(yī)大學(xué)西京醫(yī)院泌尿外科;
【基金】:國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(973)(2013CB530500) 國(guó)家自然科學(xué)基金(81372225)
【分類號(hào)】:R737.25


本文編號(hào):2224395

資料下載
論文發(fā)表

本文鏈接:http://www.wukwdryxk.cn/yixuelunwen/mjlw/2224395.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶55482***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
青青河边草直播免费观看| 人妻熟妇乱又伦精品视频中文字幕 | 国产AV无码专区亚洲A∨毛片| 男女男精品免费视频网站| 国产综合精品一区二区三区| 草草影院第一页| 亚洲精品一区二区乱码| 国产无遮挡18禁网站免费| 久久中文字幕人妻丝袜| 秋霞无码一区二区| 泗水县| 久9久综合色狠狠综合| 蜜臀久久99静品久久久久久| a网站| 日本一区二区极品视频| 国产内射爽爽大片视频社区在线 | 黑人狂桶女人高潮嗷嗷叫小说| 国产成人涩涩涩视频在线观看| 亚洲欧美日韩一区二区三区在线| 日韩av无码av免费av不卡| 济宁市| 平潭县| 亚洲欧美人成视频一区在线| 日本美女一区二区| 91中文字幕| 欧美日韩国产精品| 秋霞免费视频| 亚洲AV成人无码网站| 日韩AV人人夜夜澡人人爽| 国产亚洲大尺度无码无码专线 | 亚洲欧美在线综合色影视| 成人免费一区二区三区| 朝鲜女人大白屁股ass孕交| 亚洲线精品一区二区三区四区| 国内精品伊人久久久久AV一坑| 无码国模大尺度视频在线观看| 97久久久久人妻精品区一| 麻豆一区二区三区精品视频| 金华市| 亚洲综合无码精品一区二区三区 | 日韩久|