發(fā)布時間：2018-06-30 16:42

  本文選題：乳鼠 + 雪旺細(xì)胞　； 參考：《中國現(xiàn)代醫(yī)學(xué)雜志》2017年29期

【摘要】：目的探討乳鼠雪旺細(xì)胞對自體顱骨組織生長的影響。方法體外培養(yǎng)和純化乳鼠雪旺細(xì)胞,取乳鼠顱骨組織分為對照組和觀察組。對照組顱骨組織單獨培養(yǎng),觀察組顱骨組織與雪旺細(xì)胞共同培養(yǎng),培養(yǎng)第1、2和3周時免疫組織化學(xué)法測定顱骨組織骨鈣素和骨橋蛋白水平,酶聯(lián)免疫法吸附法測定培養(yǎng)液中骨堿性磷酸酶活性含量。結(jié)果雪旺細(xì)胞在體外被成功培養(yǎng),純度89%。不同時間點骨鈣素水平有差別(P0.05);觀察組和對照組骨鈣素水平有差別(P0.05),觀察組骨鈣素水平比對照組高;觀察組和對照組骨鈣素水平變化趨勢有差別(P0.05)。不同時間點骨橋蛋白水平無差別(P0.05);觀察組和對照組骨橋蛋白水平無差別(P0.05);觀察組和對照組骨橋蛋白水平變化趨勢無差別(P0.05)。不同時間點骨堿性磷酸酶活性有差別(P0.05);觀察組和對照組骨堿性磷酸酶活性有差別(P0.05),觀察組骨堿性磷酸酶活性比對照組高;觀察組和對照組骨堿性磷酸酶活性變化趨勢有差別(P0.05)。結(jié)論雪旺細(xì)胞可以提高顱骨組織骨鈣素水平和骨堿性磷酸酶活性,促進(jìn)顱骨組織的生長。
[Abstract]:Objective to investigate the effect of Schwann cells on the growth of autogenous skull tissue in neonatal rats. Methods Schwann cells were cultured and purified in vitro. The skulls were divided into two groups: control group and observation group. In the control group, the skull tissue was cultured alone, the observation group was co-cultured with Schwann cells, and the levels of osteocalcin and osteopontin in the skull tissue were determined by immunohistochemical method at the 2nd and 3rd week of culture. The activity of bone alkaline phosphatase in culture medium was determined by enzyme-linked immunosorbent assay (Elisa). Results Schwann cells were successfully cultured in vitro and the purity was 89%. The levels of osteocalcin in observation group and control group were different (P0.05), the levels of osteocalcin in observation group were higher than that in control group (P0.05), and the trend of osteocalcin level in observation group and control group was different (P0.05). There was no difference in osteopontin level at different time points (P0.05); there was no difference in osteopontin level between observation group and control group (P0.05); there was no difference between observation group and control group (P0.05). The activity of bone alkaline phosphatase in observation group and control group was different (P0.05), the activity of bone alkaline phosphatase in observation group was higher than that in control group (P0.05), and the change trend of bone alkaline phosphatase activity in observation group and control group was different (P0.05). Conclusion Schwann cells can increase osteocalcin level and bone alkaline phosphatase activity and promote the growth of skull tissue.
【作者單位】： 廣西醫(yī)科大學(xué)附屬口腔醫(yī)院綜合門診;廣西醫(yī)科大學(xué)附屬口腔醫(yī)院口腔種植科;
【分類號】：R318.08;R782

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