發(fā)布時(shí)間：2018-07-02 09:13

  本文選題：膠原 + 骨軟骨支架��； 參考：《中國地質(zhì)大學(xué)(北京)》2012年碩士論文

【摘要】：關(guān)節(jié)軟骨在人體中所處位置特殊,頻繁受到高強(qiáng)度外力的作用,導(dǎo)致軟骨發(fā)生損傷的幾率是很高的。但是,軟骨自身修復(fù)能力有限,一旦發(fā)生損傷病變,便很難恢復(fù),甚至還會導(dǎo)致軟骨退化。本課題利用組織工程學(xué)的基本理論,制備一種仿生多層骨軟骨支架材料。將支架材料植入受損的關(guān)節(jié)軟骨處,從而起到修復(fù)軟骨損傷的作用。 本課題選擇生物相容性良好、可降解性能優(yōu)異并在該領(lǐng)域已被廣泛使用的鼠尾膠原和殼聚糖作為支架制備的原料,配置膠原/殼聚糖混合溶液,通過冷凍干燥法使混合溶液干燥成型,作為支架材料的軟骨層。然后與本課題組成熟的聚乳酸(PLA)/骨粉骨材料結(jié)合制備得到仿生多層骨軟骨支架材料： 在掃描電鏡下分別觀察支架材料的軟骨層和骨層的形貌：結(jié)果表明,軟骨層具有既有垂直孔,又有圓孔的多層結(jié)構(gòu),骨層孔洞表面粗糙。這些形貌特點(diǎn)決定了具備這種形貌結(jié)構(gòu)的支架材料有利于細(xì)胞的附著生長。 提取小鼠的軟骨細(xì)胞,將軟骨細(xì)胞接種到多層軟骨支架材料上。觀察發(fā)現(xiàn),細(xì)胞攀爬、附著在支架的各個(gè)位置,支架材料的多層結(jié)構(gòu)對細(xì)胞的生長狀態(tài)起引導(dǎo)作用,這說明通過實(shí)驗(yàn)方法制備的支架細(xì)胞相容性良好,利于軟骨細(xì)胞的取向生長。 對制備的仿生多層骨軟骨支架材料進(jìn)行熱原性、細(xì)胞毒性、體外毒性測試、以及骨植入實(shí)驗(yàn)等生物相容性研究,證明支架材料具有良好的生物相容性,可以對軟骨損傷進(jìn)行修復(fù)。該仿生多層骨軟骨支架材料具有廣闊的應(yīng)用前景。
[Abstract]:Articular cartilage is in a special position in the human body and is frequently affected by high intensity external force, which leads to a high probability of cartilage damage. However, the repair ability of cartilage itself is limited, once damage occurs, it is difficult to recover, and even lead to cartilage degeneration. Based on the basic theory of tissue engineering, a biomimetic multilayer bone cartilage scaffold was prepared. The scaffold material is implanted into the damaged articular cartilage to repair the cartilage injury. In this paper, we choose rat tail collagen and chitosan, which have good biocompatibility, excellent biodegradability and have been widely used in this field, as raw materials for scaffold preparation, and configure collagen / chitosan mixed solution. The mixed solution was dried by freeze-drying method and used as the cartilage layer of the scaffold. Then the biomimetic multilayer bone cartilage scaffold was prepared by combining with the mature PLA / bone powder bone material. The morphology of the cartilage layer and bone layer of the scaffold was observed under scanning electron microscope. The cartilage layer has a multilayer structure of both vertical and round holes, and the surface of the holes in the bone layer is rough. These morphologies determine that scaffolds with this structure are conducive to cell adhesion and growth. The chondrocytes of mice were extracted, and the chondrocytes were inoculated into multilayer cartilage scaffolds. It was found that cell climbing and attachment to every position of the scaffold, the multilayer structure of scaffold material played a guiding role in the cell growth state, which indicated that the scaffold prepared by the experimental method had good cell compatibility. It is beneficial to the growth of chondrocytes. The biocompatibility of the biomimetic multilayer bone cartilage scaffold was studied, such as pyrogenicity, cytotoxicity, in vitro toxicity test and bone implantation experiment, which proved that the scaffold had good biocompatibility. Cartilage damage can be repaired. The biomimetic multilayer bone cartilage scaffold has a broad application prospect.
【學(xué)位授予單位】：中國地質(zhì)大學(xué)(北京)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.08

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本文編號：2089640