發(fā)布時間：2018-07-07 22:04

  本文選題：乳酸乙醇酸共聚物 + 地塞米松��； 參考：《延邊大學(xué)》2012年碩士論文

【摘要】：目的：這個研究的目的是開發(fā)一個功能性膠原蛋白膜,將涂有地塞米松作為生物活性藥物的乳酸乙醇酸共聚物(poly-lactic-co-glycolic acid, PLGA)納米顆粒的生物膜植入骨缺損區(qū),研究引導(dǎo)骨組織再生(Guided Bone Regeneration, GBR)的影響。 材料與方法：在這項研究中,含有地塞米松納米藥物顆粒,以電荷相互作用力固定于膠原蛋白膜表面。地塞米松作為生物活性藥物的PLGA納米顆粒,用water-in-oil乳液相態(tài)法制備。制作而成的納米藥物顆粒先用帶有強的正電荷的聚乙二醇(poly-ethyleneimmine, PEI)均勻涂布處理后以電荷相互作用力固定于帶有負電荷的生物膜表面。做4周的藥物釋放實驗中最初釋放率很高,之后呈一個持續(xù)性釋放。此藥物釋放系統(tǒng)在大白鼠顱骨制備了直徑為8mm的骨缺損區(qū),并進行了臨床前期的研究。植入PLGA納米藥物顆粒的可吸收性膠原蛋白膜的骨缺損區(qū)的實驗組可見,新生骨的增多及骨密質(zhì)度的增高明顯高于空白組和只植入骨移植材料(Bio-Oss(?))植入骨缺損區(qū)后,植入未作任何處理的(Bio-Gide(?))膠原蛋白膜。 結(jié)果：新型的生物活性分子釋放平臺在體內(nèi)更有效地誘導(dǎo)新骨生成,利用植入式生物膜可以對于肝細胞療法或提高在體內(nèi)的性能。 結(jié)論：新開發(fā)的PLGA納米藥物固定膠原膜系統(tǒng),是一種很有前途的適用于骨再生的材料。
[Abstract]:Objective: the aim of this study was to develop a functional collagen membrane in which poly-lactic-co-glycolic glycolate nanoparticles coated with dexamethasone as a bioactive drug were implanted into the bone defect. To study the effect of guided bone regeneration (GBR). Materials and methods: in this study, dexamethasone nanoparticles were immobilized on the surface of collagen membrane by charge interaction. Dexamethasone as a bioactive drug PLGA nanoparticles were prepared by water-in-oil emulsion phase method. The prepared nanoparticles were uniformly coated with poly-ethyleneimmine (PEI) with strong positive charge and then fixed on the surface of biofilm with negative charge by charge interaction force. After 4 weeks of drug release, the initial release rate was high, followed by a sustained release. The drug release system was used to prepare the bone defect area with diameter of 8mm in the cranium of the rat, and a preliminary clinical study was carried out. The experimental group with absorbable collagen membrane implanted PLGA nanoparticles showed that the increase of new bone and the increase of bone density were significantly higher than those of the blank group and only implanted bone graft material (Bio-Oss (?). After implantation of bone defect area, implanted without any treatment (Bio-Gide (?) Collagen membrane. Results: the new bioactive molecular release platform can induce the formation of new bone more effectively in vivo. The bioactive biofilm can be used to treat hepatocytes or improve its performance in vivo. Conclusion: the newly developed PLGA nanopharmaceutical system for collagen membrane fixation is a promising material for bone regeneration.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.08

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