發(fā)布時間：2018-07-09 17:57

  本文選題：成纖維細胞生長因子 + 神經(jīng)元　； 參考：《中國組織工程研究》2017年06期

【摘要】：背景:神經(jīng)干細胞具有自我更新和多向分化的潛能,高比例誘導其向神經(jīng)元分化具有很大的應(yīng)用前景。目的:探討堿性成纖維細胞生長因子-殼聚糖載體體外對神經(jīng)干細胞向神經(jīng)元分化的影響,及分化的神經(jīng)元與肌細胞共培養(yǎng)是否能形成突觸樣聯(lián)系。方法:純化后的神經(jīng)干細胞分別與單純殼聚糖、可溶性堿性成纖維細胞生長因子及堿性成纖維細胞生長因子-殼聚糖載體共培養(yǎng)。誘導7 d后,通過β-tubulinⅢ染色觀察向神經(jīng)元的分化;通過β-tubulinⅢ和Cha T染色觀察向膽堿能神經(jīng)元的分化;通過β-tubulinⅢ和MHC染色觀察和肌細胞之間的突觸聯(lián)系。結(jié)果與結(jié)論:堿性成纖維細胞生長因子-殼聚糖載體組,分化形成神經(jīng)元的比例是74%,顯著高于其他2組,分化的神經(jīng)元與共培養(yǎng)的肌細胞能形成突觸樣聯(lián)系。提示堿性成纖維細胞生長因子-殼聚糖載體體外能夠促進神經(jīng)干細胞的分化,且分化形成的神經(jīng)元能與共培養(yǎng)的肌細胞形成突觸樣聯(lián)系。
[Abstract]:Background: neural stem cells have the potential of self-renewal and multi-differentiation. Aim: to investigate the effect of basic fibroblast growth factor-chitosan carrier on neural stem cell differentiation into neurons in vitro and whether co-culture of differentiated neurons and myocytes can form synaptic connections. Methods: purified neural stem cells were co-cultured with chitosan, soluble basic fibroblast growth factor and basic fibroblast growth factor-chitosan carrier respectively. After 7 days of induction, the differentiation to neurons was observed by 尾 -tubulin 鈪，

本文編號：2110163