發(fā)布時(shí)間：2018-12-16 07:55

【摘要】：目的探討前列腺干細(xì)胞抗原基因(PSCA)和磷脂酶(PL)CE1基因多態(tài)性與老年幽門(mén)螺旋桿菌(Hp)感染胃潰瘍的易感性及對(duì)臨床預(yù)后的影響。方法采用病例-對(duì)照研究設(shè)計(jì),選取130例胃潰瘍并且Hp檢查為陽(yáng)性的老年患者作為病例組,另外按性別和年齡匹配的胃潰瘍并且Hp檢查為陰性的老年患者作為對(duì)照組。所有患者治療前抽取外周靜脈血并提取DNA,設(shè)計(jì)PSCA(rs2294008)和PLCE1(rs11599672)聚合酶鏈反應(yīng)(PCR)引物進(jìn)行PCR擴(kuò)增,擴(kuò)增產(chǎn)物采用DNA測(cè)序分析PSCA和PLCE1基因類(lèi)型,采用Logistic回歸計(jì)算校正相對(duì)危險(xiǎn)度(OR)和95%置信區(qū)間(95%CI)評(píng)價(jià)PSCA和PLCE1基因多態(tài)性與老年Hp感染胃潰瘍的易感性。結(jié)果 1兩組年齡、性別、病程、病變部位差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);2與rs2294008基因型CC比較,基因型為CT、TT和CT/TT的患者Hp感染危險(xiǎn)性降低(P0.01);3與PLCE1 rs11599672基因型AA比較,基因型為AC、CC和AC/CC的患者Hp感染危險(xiǎn)性降低(P0.05);4不同PSCA多態(tài)位點(diǎn)rs2294008和LCEl多態(tài)位點(diǎn)rs11599672患者的Hp根除率和潰瘍愈合率差異具有統(tǒng)計(jì)意義(P0.01)。結(jié)論 PSCA rs2294008基因型CC和PLCE1 rs11599672基因型AA可增加Hp感染胃潰瘍易感性,并且顯著降低臨床預(yù)后。
[Abstract]:Objective to investigate the relationship between polymorphisms of prostate stem cell antigen (PSCA) and phospholipase (PL) CE1) genes and the susceptibility to gastric ulcer infection by Helicobacter pylori (Hp) in the elderly and its influence on clinical prognosis. Methods A case-control study was carried out in which 130 patients with gastric ulcer and positive Hp were selected as the case group, and the patients with gastric ulcer matched by sex and age with negative Hp were selected as the control group. Peripheral venous blood was extracted from all patients before treatment and DNA, was extracted to design PSCA (rs2294008) and PLCE1 (rs11599672) polymerase chain reaction (PCR) primers for PCR amplification. PSCA and PLCE1 gene types were analyzed by DNA sequencing. Corrected relative risk (OR) and 95% confidence interval (95%CI) were calculated by Logistic regression to evaluate the susceptibility of PSCA and PLCE1 gene polymorphisms to gastric ulcer infection in elderly Hp patients. Results (1) there was no significant difference in age, sex, course of disease and lesion site between the two groups (P0.05), 2 compared with rs2294008 genotype CC, the risk of Hp infection in patients with CT,TT and CT/TT genotypes was lower (P0.01). 3Compared with PLCE1 rs11599672 genotype AA, the risk of Hp infection in patients with genotype AC,CC and AC/CC was lower (P0.05). 4There were significant differences in Hp eradication rate and ulcer healing rate between rs2294008 and LCEl polymorphic rs11599672 patients with different PSCA polymorphic loci (P0.01). Conclusion PSCA rs2294008 genotype CC and PLCE1 rs11599672 genotype AA can increase the susceptibility of Hp to gastric ulcer infection and significantly reduce the clinical prognosis.
【作者單位】： 聊城市人民醫(yī)院;聊城市復(fù)退軍人醫(yī)院;
【基金】：山東省自然科學(xué)基金聯(lián)合專(zhuān)項(xiàng)(No.ZR2014HL019)
【分類(lèi)號(hào)】：R573

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本文編號(hào)：2382024