發(fā)布時間：2018-03-02 14:44

  本文選題：POUF基因　切入點：基因多態(tài)位點　出處：《西南農(nóng)業(yè)學(xué)報》2017年07期 　論文類型：期刊論文

【摘要】：【目的】為探明荔波瑤山雞POU1F1基因多態(tài)性與繁殖性狀的關(guān)系,為荔波瑤山雞的選擇性育種提供參考。【方法】針對POU1F1基因設(shè)計6對引物,采用PCR測序法篩選出多態(tài)位點,并利用荔波瑤山雞母系60個個體進行繁殖性狀的關(guān)聯(lián)分析。【結(jié)果】荔波瑤山雞POU1F1基因有8個多態(tài)位點,其中POU1F1基因58(A→G)與300日齡產(chǎn)蛋量、首次就巢日齡、300日齡就巢次數(shù)均呈顯著或極顯著相關(guān)(P0.05或P0.01);65(T→C)與300日齡產(chǎn)蛋量、首次就巢日齡、300日齡就巢次數(shù)呈顯著相關(guān)(P0.05);11041(T→C)與300日齡產(chǎn)蛋量、首次就巢持續(xù)時間、首次就巢至重新開產(chǎn)間隔均呈顯著或極顯著相關(guān)(P0.05或P0.01)�！窘Y(jié)論】POU1F1基因58(A→G)和65(T→C)可作為300日齡產(chǎn)蛋量、首次就巢日齡和300日齡就巢次數(shù)的輔助選擇標記;11041(T→C)可作為300日齡產(chǎn)蛋量、首次就巢持續(xù)時間、首次就巢至重新開產(chǎn)間隔的輔助選擇標記。
[Abstract]:[objective] to investigate the relationship between POU1F1 gene polymorphism and reproductive traits in Libo Yao Shan Chicken, and to provide reference for selective breeding of Libo Yao Chicken. [methods] six pairs of primers were designed for POU1F1 gene, and polymorphic loci were screened by PCR sequencing. The reproductive traits of Libo Yaoshan Chicken were analyzed by association analysis of 60 individuals. [results] there were 8 polymorphic loci in the POU1F1 gene of Libo Yaoshan Chicken, among which POU1F1 gene 58A. 鈫扜) was significantly or extremely significantly correlated with egg production at 300 days of age and the number of nests at 300 days of age for the first time (P 0.05 or P 0.01). 鈫扖) was significantly correlated with the number of eggs laid at 300 days of age and the number of nests at 300 days of age for the first time (P0.05 / 11041T). 鈫扖) was significantly correlated with egg production at 300 days of age, first nest duration, first nest to reopening interval (P0.05 or P0.01A). [conclusion] POU1F1 gene 58A. 鈫扜) and 65T. 鈫扖) can be used as an auxiliary selection marker for egg production at 300 days of age, and for the first time, 11041T for the number of nests at the age of 300 days. 鈫扖) can be used as an auxiliary selection marker for egg production at 300 days of age, for the first time for the duration of the nest, and for the first time from the nest to the reopening interval.
【作者單位】： 貴州省畜牧獸醫(yī)研究所;
【基金】：貴州省聯(lián)合基金項目“瑤山雞ONECUT1基因的CDS區(qū)克隆;差異表達及與生產(chǎn)性狀關(guān)聯(lián)分析”[黔科合LH字(2015)7067] 貴州省基金項目“瑤山雞就巢性選育關(guān)鍵基因篩選”[黔科合J字(2014)2102] 貴州省農(nóng)業(yè)動植物育種項目“瑤山雞新品系選育與推廣”[黔農(nóng)育專字(2014)008] 貴州省農(nóng)業(yè)科學(xué)院2014年博士基金項目“瑤山雞STAT5基因變異檢測及與產(chǎn)蛋性狀關(guān)聯(lián)分析” 貴州省農(nóng)業(yè)科學(xué)院院專項“長順綠殼蛋雞綠殼表型遺傳標記的篩選與應(yīng)用”[黔農(nóng)科院院專項(2014)006],“百宜黑雞高產(chǎn)純繁核心種群選育與示范推廣”[黔農(nóng)科院院專項(2014)038] 市院地合作項目“喀斯特地形山地肉雞養(yǎng)殖技術(shù)推廣”[院地農(nóng)科合字(2014)10]
【分類號】：S831

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