Panx1基因過表達(dá)對(duì)人肝癌Hep3B細(xì)胞增殖及遷移的影響
發(fā)布時(shí)間：2018-10-29 20:38
【摘要】：目的探討Panx1基因過表達(dá)對(duì)人肝癌Hep3B細(xì)胞增殖及遷移的影響。方法構(gòu)建Panx1過表達(dá)慢病毒質(zhì)粒和過表達(dá)空載慢病毒質(zhì)粒,包裝生產(chǎn)慢病毒,分別轉(zhuǎn)染Hep3B細(xì)胞(過表達(dá)組、對(duì)照組)。用Western blotting法檢測Hep3B細(xì)胞Panx1、上皮鈣黏蛋白(E-cadherin)及波形蛋白(Vimentin)表達(dá),CCK-8法檢測細(xì)胞增殖能力(OD值),劃痕實(shí)驗(yàn)檢測細(xì)胞遷移能力(相對(duì)遷移距離、劃痕愈合率)。結(jié)果過表達(dá)組Panx1、E-cadherin蛋白相對(duì)表達(dá)量較對(duì)照組高(P均0.05),Vimentin蛋白相對(duì)表達(dá)量較對(duì)照組低(P0.05);各時(shí)間點(diǎn)(0 h除外)OD值較對(duì)照組低(P均0.05);相對(duì)遷移距離較對(duì)照組低,劃痕愈合率較對(duì)照組高(P均0.05)。結(jié)論 Panx1基因過表達(dá)可抑制Hep3B細(xì)胞增殖與遷移。
[Abstract]:Objective to investigate the effect of overexpression of Panx1 gene on proliferation and migration of human hepatoma Hep3B cells. Methods Panx1 overexpression lentivirus plasmids and empty lentivirus plasmids were constructed and packaged to produce lentivirus. The lentivirus was transfected into Hep3B cells (overexpression group, control group). The expression of Panx1, epithelial cadherin (E-cadherin) and vimentin (Vimentin) was detected by Western blotting assay, cell proliferation ability (OD value) was detected by CCK-8 assay, and migration ability (relative migration distance, scratch healing rate) was detected by scratch test. Results the relative expression of Panx1,E-cadherin protein in the overexpression group was higher than that in the control group (P < 0. 05), the OD value at each time point (except 0 h) was lower than that in the control group (P 0. 05), and the relative expression of), Vimentin protein in the overexpression group was lower than that in the control group (P 0. 05). The relative migration distance was lower than that of control group, and the rate of scratch healing was higher than that of control group (P 0.05). Conclusion overexpression of Panx1 gene can inhibit the proliferation and migration of Hep3B cells.
【作者單位】： 青島大學(xué)醫(yī)學(xué)院附屬青島市市立醫(yī)院;
【分類號(hào)】：R735.7
本文編號(hào)：2298752
[Abstract]:Objective to investigate the effect of overexpression of Panx1 gene on proliferation and migration of human hepatoma Hep3B cells. Methods Panx1 overexpression lentivirus plasmids and empty lentivirus plasmids were constructed and packaged to produce lentivirus. The lentivirus was transfected into Hep3B cells (overexpression group, control group). The expression of Panx1, epithelial cadherin (E-cadherin) and vimentin (Vimentin) was detected by Western blotting assay, cell proliferation ability (OD value) was detected by CCK-8 assay, and migration ability (relative migration distance, scratch healing rate) was detected by scratch test. Results the relative expression of Panx1,E-cadherin protein in the overexpression group was higher than that in the control group (P < 0. 05), the OD value at each time point (except 0 h) was lower than that in the control group (P 0. 05), and the relative expression of), Vimentin protein in the overexpression group was lower than that in the control group (P 0. 05). The relative migration distance was lower than that of control group, and the rate of scratch healing was higher than that of control group (P 0.05). Conclusion overexpression of Panx1 gene can inhibit the proliferation and migration of Hep3B cells.
【作者單位】： 青島大學(xué)醫(yī)學(xué)院附屬青島市市立醫(yī)院;
【分類號(hào)】：R735.7
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