發(fā)布時(shí)間：2018-11-04 17:12

【摘要】：以唐菖蒲(Gladiolus hybridus)品種"Rose Supreme"為材料,采用hiTAIL-PCR方法克隆得到GhMYC2基因上游906bp的啟動(dòng)子序列。利用植物順式作用元件查詢數(shù)據(jù)庫PLACE分析發(fā)現(xiàn),GhMYC2啟動(dòng)子序列中不僅含有響應(yīng)茉莉酸(jasmonate,JA)的元件T/GBOXATPIN2,還含有大量與病原菌誘發(fā)因子表達(dá)調(diào)節(jié)相關(guān)的轉(zhuǎn)錄因子WRKY以及抗性元件W-box、E-box等順式作用元件。在ProGhMYC2::GUS轉(zhuǎn)基因擬南芥中發(fā)現(xiàn),GhMYC2的表達(dá)具有時(shí)空特異性,且主要在根、葉中表達(dá)。對(duì)轉(zhuǎn)基因擬南芥進(jìn)行茉莉酸甲酯(methyl jasmonic acid,MeJA)和灰霉菌(Botrytis cinerea)處理發(fā)現(xiàn),轉(zhuǎn)基因植株中GUS的表達(dá)均上調(diào),表明其表達(dá)受到MeJA和灰霉菌的影響,此結(jié)果可為研究JAs途徑調(diào)控植物抵御病害提供一定的參考。
[Abstract]:Using Gladiolus (Gladiolus hybridus) variety "Rose Supreme" as the material, the promoter sequence of GhMYC2 gene upstream 906bp was cloned by hiTAIL-PCR method. The PLACE analysis of plant cis-acting element query database showed that the GhMYC2 promoter sequence not only contains the element T / GBOX ATP IN2, which is responsive to jasmonate,JA, but also contains the component T / GBOX ATP-IN2, which is responsible for jasmonic acid (jasmonate,JA). There are also a large number of transcription factor WRKY related to the expression regulation of pathogen inducing factors and cis-acting elements such as the resistance element W-box E-box and so on. In ProGhMYC2::GUS transgenic Arabidopsis thaliana, it was found that the expression of GhMYC2 was space-time specific and mainly expressed in roots and leaves. The expression of GUS was up-regulated in transgenic Arabidopsis thaliana treated with methyl jasmonate (methyl jasmonic acid,MeJA) and (Botrytis cinerea), indicating that the expression of GUS was affected by MeJA and Ash. These results may provide some references for the study of plant disease resistance by JAs pathway.
【作者單位】： 中國農(nóng)業(yè)大學(xué)園藝學(xué)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(31171991) 高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金資助項(xiàng)目(20120008110006)
【分類號(hào)】：Q943.2;S682.24
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本文編號(hào)：2310572