發(fā)布時(shí)間：2018-06-17 10:31

  本文選題：流感疫苗 + 殼聚糖��； 參考：《吉林大學(xué)》2007年碩士論文

【摘要】： 目前預(yù)防流行感冒的主要手段是注射流感疫苗,基于目前對(duì)鼻腔免疫和新藥載體的研究,為流感病毒殼聚糖微球鼻腔免疫疫苗的研究提供了可能,用殼聚糖包封流感病毒,通化鼻腔給藥,達(dá)到免疫目的,避免注射痛觸,提高生物利用度。 研究發(fā)現(xiàn)選擇TPP終濃度為400ug/ml,固化比為1:5,pH為5.6作為殼聚糖/流感病毒微球的制備條件,可制成粒徑小于10um的微球。還進(jìn)一步對(duì)殼聚糖/流感全病毒微粒鼻腔免疫進(jìn)行初步研究,選6-8周的昆明鼠18只分三組,分別為殼聚糖/流感病毒樣品組,流感病毒組,空白微球組,所有小鼠免疫3周后加強(qiáng)免疫一次。第一次免疫后第3周、第6周、第9周、12周采集血液和鼻道沖洗液。用間接ELISA方法檢測(cè)血清中IgG和鼻道沖洗液中的IgA。實(shí)驗(yàn)結(jié)果表明殼聚糖/流感病毒能夠在遠(yuǎn)端粘膜位點(diǎn)分泌較強(qiáng)的IgA,這對(duì)于通過呼吸道感染的流感病毒的預(yù)防十分重要,盡管其有效性還需要大量的實(shí)驗(yàn)證明,本論文說明了殼聚糖微球作為流感鼻腔免疫的可行性。
[Abstract]:At present, the main means of preventing influenza is to inject influenza vaccine. Based on the current research on nasal immunization and new drug carriers, it is possible to study the vaccine against influenza virus by chitosan microspheres. Tonghua nasal administration to achieve the purpose of immunity, avoid injection of pain, improve bioavailability. It was found that when the final concentration of TPP was 400ugml and the curing ratio was 1: 5g / ml, pH 5.6 was used as the preparation condition of chitosan / influenza virus microspheres, the microspheres with diameter smaller than 10um could be prepared. A preliminary study on nasal immunization of chitosan / influenza virus particles was carried out. Eighteen Kunming mice aged 6-8 weeks were divided into three groups: chitosan / influenza virus sample group, influenza virus group, and blank microsphere group. All mice were immunized once after 3 weeks. Blood and nasal irrigation fluid were collected 3 weeks, 6 weeks, 9 weeks and 12 weeks after the first immunization. IgG in serum and IgA in nasal irrigation fluid were detected by indirect Elisa. The results show that chitosan / influenza virus can secrete strong IgA at the distal mucosal site, which is very important for the prevention of influenza virus infected by respiratory tract, although its effectiveness needs a lot of experimental proof. In this paper, the feasibility of chitosan microspheres as nasal immunity for influenza was demonstrated.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R392

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本文編號(hào)：2030730