發(fā)布時(shí)間：2018-06-19 13:13

  本文選題：嗅球 + 嗅鞘細(xì)胞　； 參考：《青島大學(xué)》2006年碩士論文

【摘要】： 【目的】獲得較為理想的取材和純化嗅鞘細(xì)胞的方法措施。 【方法】通過(guò)對(duì)多種方法的比較獲得較為理想純化嗅鞘細(xì)胞的措施，嗅球取材直接擠壓法，將細(xì)胞接種密度統(tǒng)一調(diào)整到10~5／ml后應(yīng)用3種純化方法各對(duì)10份樣本處理并作純度比較；同樣密度和接種數(shù)量的原代細(xì)胞各取10份種植于3種不同的培養(yǎng)皿，對(duì)細(xì)胞貼壁速度和最終純度進(jìn)行檢驗(yàn)。最好的純化嗅鞘細(xì)胞方法為免疫吸附法，其細(xì)胞純度達(dá)84.66％。而差速貼壁法(71.59％)和化學(xué)試劑法(66.45％)均由于損失細(xì)胞太多不可取；同時(shí)發(fā)現(xiàn)包被培養(yǎng)皿對(duì)純化細(xì)胞意義重大，P~(75)包被組不僅培養(yǎng)細(xì)胞的純度最高(88.69％)，而且還有利于OECs的快速貼壁。 【結(jié)果】我們培養(yǎng)人胚嗅鞘細(xì)胞純度為84.66％，同時(shí)發(fā)現(xiàn)包被培養(yǎng)皿對(duì)純化細(xì)胞意義重大，，p75包被不僅培養(yǎng)細(xì)胞的純度高，而且還有利于OECs的快速貼壁。 【結(jié)論】此取材及培養(yǎng)方法收獲大量嗅鞘細(xì)胞將對(duì)其進(jìn)一步的基礎(chǔ)研究以及將來(lái)應(yīng)用于臨床奠定基礎(chǔ)。
[Abstract]:Objective : To obtain a better method for obtaining and purifying olfactory ensheathing cells .






Through the comparison of various methods , the method can purify olfactory ensheathing cells , direct extrusion of olfactory bulb , adjust the inoculation density of cells to 10 ~ 5 / ml , and compare the purity of 10 samples with 3 kinds of purification methods .
The best purified olfactory ensheathing cells were immunosorbed , the purity of the cells was 84.66 % , and the differential adherent method ( 71.59 % ) and the chemical reagent method ( 66.45 % ) were not desirable because of the loss of cells .
At the same time , it was found that the culture dish was of great significance to purify the cells , but not only the purity of the cultured cells was the highest ( 88.69 % ) , but also the rapid adherent of OECs was also facilitated .






The results showed that the purity of cultured human embryonic olfactory ensheathing cells was 84.66 % , while it was found that the coated culture dish was of great significance to the purified cells . The p75 coating was not only the high purity of cultured cells but also the rapid adherent of OECs .






Conclusion The harvesting of olfactory ensheathing cells from this material and culture method will provide a foundation for further basic research and clinical application in the future .
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R329

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本文編號(hào)：2040006