發(fā)布時間：2018-03-08 15:46

  本文選題：流感病毒　切入點：金剛烷胺　出處：《廣西大學》2015年碩士論文　論文類型：學位論文

【摘要】：本研究使用氨基酸耐藥性位點分析與微量細胞病變抑制法相結合的方法對廣西區(qū)近年來18株豬流感病毒H1N1和1株馬流感病毒H3N8亞型進行耐藥性研究。通過對這些毒株進行NA與M基因的克隆測序,基因序列的比對,序列分析結果顯示,18株豬流感病毒H1N1亞型的M2基因耐藥性位點上均發(fā)生S31N突變；1株馬H3的毒株耐藥性位點沒有發(fā)生突變。在NA酶活性位點及周圍相關位點上,廣西區(qū)近年來18株豬流感病毒H1N1毒株中,GX3發(fā)生了E119K的突變,6號發(fā)生E119G突變,G2發(fā)生N295D突變,其余的15株H1N1流感病毒均無發(fā)生突變；馬流感H3N8毒株發(fā)生S247A突變。微量細胞病變抑制法實驗中,使用濃度為1mg/ml的鹽酸金剛烷胺能抑制毒株馬H3感染MDCK細胞,該毒株對該濃度下的鹽酸金剛烷胺敏感,藥物有效濃度為10-1.5 mg/ml；18株豬流感病毒對該濃度的鹽酸金剛烷胺耐藥,MDCK細胞均被感染,感染后發(fā)生病變。微量細胞病變抑制法實驗中,使用濃度為1mg/,l的磷酸奧司他韋,能抑制18株豬流感病毒感染MDCK細胞,這些毒株對該濃度下的磷酸奧司他韋敏感,藥物有效濃度在10-1～10-2.76mg/ml之間；毒株馬H3對該濃度的磷酸奧司他韋耐藥,MDCK細胞在被感染后發(fā)生細胞病變。
[Abstract]:In this study, amino acid resistance site analysis combined with microcytopathic inhibition was used to study the resistance of 18 swine influenza viruses (H1N1) and 1 equine influenza virus (H3N8) subtypes in Guangxi in recent years. Na and M genes were cloned and sequenced. The results of sequence analysis showed that S31N mutation was not found in the resistant loci of M2 gene of swine influenza virus H1N1 subtype and that of horse H3 strain H3 strain, and no mutation was found at the Na-enzyme activity site and the related loci around the Swine Influenza virus H1N1 subtype, the results of sequence analysis showed that there was no mutation in the resistance loci of S31N mutation. In recent years, the mutation of E119K was found in GX3 of 18 swine influenza virus H1N1 strains in Guangxi, N295D mutation occurred in G _ 2 of G _ 2 mutation of E119G mutation on 6, and no mutation was found in the other 15 strains of H1N1 influenza virus. In the microcytopathic inhibition assay, a dose of 1 mg / ml of amantadine hydrochloride could inhibit the infection of equine H3 to MDCK cells, and the strain was sensitive to amantadine hydrochloride at that concentration. The effective concentration of the drug was 10-1.5 mg / ml ~ (-1) mg / ml ~ (-1) mg 路ml ~ (-1) 路ml ~ (-1) mg 路ml ~ (-1) 路ml ~ (-1) mg 路ml ~ (-1) mg 路ml ~ (-1) mg 路ml ~ (-1) mg 路ml ~ (-1) mg 路ml ~ (-1) mg / L of oseltamivir phosphate. These strains were sensitive to oseltamivir phosphate at that concentration, and the effective concentration of the drug was between 10-1 and 10-2.76 mg / ml. Cytopathic effect of horse H _ 3 strain on oseltamivir phosphate resistant MDCK cells was observed after infection.
【學位授予單位】：廣西大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S852.65
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本文編號：1584521