a国产,中文字幕久久波多野结衣AV,欧美粗大猛烈老熟妇,女人av天堂

脫氧雪腐鐮刀菌烯醇的環(huán)七肽模擬表位篩選

發(fā)布時(shí)間:2019-06-14 12:04
【摘要】:利用噬菌體隨機(jī)環(huán)七肽庫(kù)篩選脫氧雪腐鐮刀菌烯醇(Deoxynivalenol,DON)模擬表位。以DON單抗為靶分子,四輪固相淘選噬菌體隨機(jī)環(huán)七肽庫(kù),篩選出陽(yáng)性噬菌體,并通過ELISA實(shí)驗(yàn)檢測(cè)其特異性。對(duì)陽(yáng)性克隆的DNA進(jìn)行測(cè)序,取序列不同的陽(yáng)性克隆,分別建立陽(yáng)性噬菌體與DON標(biāo)準(zhǔn)品的競(jìng)爭(zhēng)抑制曲線。對(duì)30個(gè)噬菌體單克隆進(jìn)行活性測(cè)定,結(jié)果顯示能與DON單抗結(jié)合的噬菌體克隆有28株,其中的22株能被DON標(biāo)準(zhǔn)品阻斷與DON單抗結(jié)合。DNA測(cè)序結(jié)果顯示,21株陽(yáng)性噬菌體的序列是PFPNHPY,另一株的序列是TPWTQHL。其相應(yīng)噬菌體與DON毒素標(biāo)準(zhǔn)品的競(jìng)爭(zhēng)曲線結(jié)果顯示,8號(hào)噬菌體建立的競(jìng)爭(zhēng)抑制曲線線性范圍是0.0292~0.636μg/mL,IC_(50)為0.169μg/mL;4號(hào)噬菌體建立的競(jìng)爭(zhēng)抑制曲線線性范圍是0.0124~0.271μg/mL,IC_(50)為0.058μg/mL。在隨機(jī)環(huán)七肽庫(kù)中篩選到TPWTQHL和PFPNHPY兩個(gè)DON模擬表位,可替代DON毒素標(biāo)準(zhǔn)品,建立DON的免疫學(xué)無(wú)毒檢測(cè)技術(shù)。
[Abstract]:The simulated epitopes of deoxynivalenol (Deoxynivalenol,DON) were screened by bacteriophage random cyclic seven-peptide library. Using DON monoclonal antibody as the target molecule, the random cyclic seven peptide library of bacteriophage was selected by four rounds of solid phase scouring, and the positive bacteriophage was screened out, and its specificity was detected by ELISA assay. The DNA of the positive clone was sequenced, and the competitive inhibition curves of the positive bacteriophage and the DON standard were established by taking the positive clones with different sequences. The activity of 30 bacteriophage monoclonal antibodies was determined. the results showed that there were 28 bacteriophage clones that could bind to DON monoclonal antibodies, 22 of which could be blocked by DON standard to bind to DON monoclonal antibodies. DNA sequencing results showed that the sequence of 21 positive bacteriophages was the sequence of the other strain of PFPNHPY, was TPWTQHL.. The results of the competition curve between the corresponding bacteriophage and DON toxin standard showed that the linear range of competitive inhibition curve established by bacteriophage 8 was 0.0292 ~ 0.636 渭 g / mL,IC_ (50). The linear range of competitive inhibition curve established by bacteriophage 0.169 渭 g / mL;4 was 0.0124 ~ 0.271 渭 g / mL,IC_ (50). 0.058 渭 g / mL.. Two DON mimic epitopes, TPWTQHL and PFPNHPY, were screened from the random cyclic seven peptide library, which can replace the standard of DON toxin and establish the immunological nontoxic detection technique of DON.
【作者單位】: 暨南大學(xué)生命科學(xué)技術(shù)學(xué)院生物工程學(xué)系;
【基金】:“十一五”國(guó)家科技支撐計(jì)劃項(xiàng)目(2008BAK42B05)
【分類號(hào)】:S816.17;TS210.7

【相似文獻(xiàn)】

相關(guān)期刊論文 前4條

1 徐玲;黃鳳丹;彭思璐;劉仁榮;裘雪梅;陳興龍;;黃曲霉毒素B_1抗原模擬表位高效表達(dá)[J];中國(guó)公共衛(wèi)生;2013年02期

2 熊爭(zhēng)平;許楊;何慶華;;NFLX抗原模擬表位的淘選及鑒定[J];南昌大學(xué)學(xué)報(bào)(理科版);2013年04期

3 陳興龍;徐玲;劉仁榮;裘雪梅;朱立鑫;;絲狀噬菌體pⅧ展示系統(tǒng)表達(dá)赭曲霉毒素A模擬表位及應(yīng)用研究[J];衛(wèi)生研究;2012年05期

4 陳興龍;徐玲;劉仁榮;裘雪梅;朱立鑫;;基于噬菌體展示技術(shù)的赭曲霉毒素A高密度模擬表位的表達(dá)研究[J];食品科學(xué);2012年19期

相關(guān)博士學(xué)位論文 前1條

1 何慶華;基于模擬表位的玉米赤霉烯酮全抗原生物合成及其免疫學(xué)檢測(cè)性能的研究[D];南昌大學(xué);2012年

相關(guān)碩士學(xué)位論文 前2條

1 熊爭(zhēng)平;諾氟沙星模擬表位的淘選、應(yīng)用及針對(duì)小分子噬菌體隨機(jī)肽庫(kù)的建立[D];南昌大學(xué);2014年

2 徐富勇;DON模擬表位的兩種表達(dá)載體的構(gòu)建及應(yīng)用研究[D];南昌大學(xué);2013年

,

本文編號(hào):2499368

資料下載
論文發(fā)表

本文鏈接:http://www.wukwdryxk.cn/yixuelunwen/dongwuyixue/2499368.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶092f1***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
91天堂| 亚洲VA在线VA天堂XXXX中文| 天堂在线最新版资源www| 大理市| 四虎电影院| 91久久精品www人人做人人爽| 熟女少妇av| 亚洲av在线| 张家界市| 精品久久99| 人妻少妇系列| 久久久久久黄色| 秦安县| 四虎国产精品亚洲一区久久特色| 日本免费一区二区三区| 人妻无码久久精品人妻| 国产精品偷伦视频免费观看了| 顶的速度越来越快越| 无码丰满熟妇juliaann与黑人| 人妻无码一区二区三区| 永久免费AV无码不卡在线观看| 日韩精品人妻无码久久影院| 亚洲国产aⅴ综合网| 少妇精品揄拍高潮少妇| 亚洲国产成人精品无码区二本| 草木影视在线视频免费观看| 国产乱子伦精品中文| 亚洲综合图| 亚洲国产精品国自产拍久久 | 通道| 国产激情综合在线看| 精品无码av人在线观看| 亚洲国产精品无码中文字满| 色偷拍 自怕 亚洲 10P| 精品国内自产拍在线观看| 久久99国产综合精品| 久久人人爽人人爽av片| 99久久精品国产综合| 久久aⅴ无码av高潮白浆心得| 亚洲国产一区二区三区| 欧美午夜特黄aaaaaa片|