發(fā)布時(shí)間：2019-06-26 23:15

【摘要】：恩拉霉素是由放線菌(Streptomyces fungicidicus)發(fā)酵而得的一類多肽類抗生素,由于其獨(dú)特的分子結(jié)構(gòu),對(duì)革蘭氏陽(yáng)性菌有很強(qiáng)的活性,特別是對(duì)腸道內(nèi)的有害梭菌有很強(qiáng)的抑殺作用。作為全新的飼料添加劑,恩拉霉素能促進(jìn)豬、雞等的生長(zhǎng),廣泛用于畜牧業(yè)。目前恩拉霉素的含量測(cè)定是按照農(nóng)業(yè)部1999年頒發(fā)的《進(jìn)口獸藥質(zhì)量標(biāo)準(zhǔn)》方法進(jìn)行,本研究根據(jù)恩拉霉素的結(jié)構(gòu)特點(diǎn),通過(guò)實(shí)驗(yàn),建立了恩拉霉素的高效液相色譜檢測(cè)法。篩選優(yōu)化色譜條件為:色譜柱:Thermo C18,5μm,4.6mm×250mm;流動(dòng)相:乙腈:0.05mol/L磷酸二氫鈉溶液=30:70;紫外檢測(cè)波長(zhǎng)267nm;柱溫:30℃;流速:1.0mL/min;進(jìn)樣量:20μL。在該條件下,檢測(cè)結(jié)果顯示恩拉霉素A和恩拉霉素B在12.5~200μg/mL范圍內(nèi)線性關(guān)系良好,恩拉霉素A和恩拉霉素B的回歸方程分別為A1=11587x+18926(R2=0.998)和A2=35969x-46745(R2=0.996);恩拉霉素A的平均回收率為98.9%,恩拉霉素B的平均回收率為98.7%;相對(duì)標(biāo)準(zhǔn)偏差(RSD)為0.27%。實(shí)驗(yàn)結(jié)果證明采用建立的高效液相色譜法(HPLC)檢測(cè)恩拉霉素含量具有線性范圍寬、分析時(shí)間短、樣品前處理簡(jiǎn)便、定量結(jié)果準(zhǔn)確、重復(fù)性好、穩(wěn)定性好和回收率高等特點(diǎn),可以作為恩拉霉素含量檢測(cè)的質(zhì)控方法。同時(shí)將高效液相色譜法與現(xiàn)行標(biāo)準(zhǔn)微生物檢測(cè)法進(jìn)行了比較,進(jìn)一步證明高效液相色譜法(HPLC)操作簡(jiǎn)便、快速、精準(zhǔn)的特點(diǎn),為其質(zhì)量控制提供了更為可靠的依據(jù)。
[Abstract]:Enramycin is a kind of polypeptide antibiotics fermented by actinomycetes (Streptomyces fungicidicus). Because of its unique molecular structure, it has strong activity against Gram-positive bacteria, especially against harmful Clostridium perfringens in intestinal tract. As a new feed additive, Enramycin can promote the growth of pigs, chickens and so on, and is widely used in animal husbandry. At present, the content of Enramycin was determined according to the quality Standard of imported Veterinary drugs issued by the Ministry of Agriculture in 1999. according to the structural characteristics of Enramycin, a high performance liquid chromatography (HPLC) method for the determination of Enramycin was established. The optimum chromatographic conditions were as follows: chromatographic column: Thermo C 18, 5 渭 m, 4.6mm 脳 250 mm, mobile phase: acetonitrile: 0.05mol/L sodium dihydrogen phosphate solution = 30 鈮，

本文編號(hào)：2506583