發(fā)布時間：2018-06-05 00:17

  本文選題：骨髓基質干細胞 + 誘導分化��； 參考：《蘇州大學》2009年碩士論文

【摘要】： 第一部分還原型谷胱甘肽誘導大鼠骨髓基質干細胞分化過程中NSE、GFAP相關mRNA及蛋白表達變化研究 目的:初步探討還原型谷胱甘肽(GSH)對大鼠骨髓基質干細胞(bone marrow stromalcells,BMSCs)的誘導分化機制。 方法:體外培養(yǎng)和純化大鼠BMSCs,用GSH對其進行誘導,分別于誘導后6h、12h、24h后對其進行實時定量PCR和Westblot檢測,測定各組中神經(jīng)元特異性烯醇化酶(neuron specific enolase,NSE)和膠質纖維酸性蛋白(glial fibrillary acidic protein,GFAP)mRNA及蛋白水平的表達情況。 結果:誘導6h、12h、24h后BMSCs分化形成神經(jīng)元樣細胞和膠質細胞樣細胞,實時定量PCR和Westblot檢測結果表明BMSCs中NSE及GFAP的mRNA與蛋白水平表達明顯高于對照組,以12h最為明顯。 結論:GSH可以誘導BMSCs分化形成神經(jīng)元樣細胞和膠質細胞樣細胞,且誘導后NSE及GFAP的mRNA與蛋白水平表達增高。 第二部分堿性成纖維細胞生長因子與表皮生長因子誘導大鼠骨髓基質干細胞向神經(jīng)元樣細胞分化相關基因表達譜研究 目的:初步探討大鼠骨髓基質干細胞(BMSCs)向神經(jīng)元樣細胞分化前后基因表達譜的變化,尤其是與神經(jīng)元發(fā)育相關基因的表達情況。 方法:體外培養(yǎng)和純化大鼠BMSCs,用堿性成纖維細胞生長因子(basic fibroblastgrowth factor,bFGF)和表皮生長因子(epidermal growth factor,EGF)對其進行誘導,分別于誘導前和誘導后第七天用Affymetrix大鼠表達譜芯片檢測,并且利用生物信息學分析技術比較誘導前后基因表達譜的改變。 結果:共有差異基因216條,其中上調基因125條,下調基因91條.與神經(jīng)元發(fā)育密切相關的基因有13條,其中上調7條,下調6條。 結論:經(jīng)體外誘導的BMSCs具有向神經(jīng)元分化的潛能并能表達一些與神經(jīng)元分化有關的重要基因。這些基因將是后續(xù)研究的重點對象。
[Abstract]:Part I changes in NSE, GFAP related mRNA and protein expression in rat bone marrow stromal cells induced by reduced glutathione
Objective: To explore the mechanism of reduced glutathione (GSH) induced differentiation of bone marrow stromalcells (BMSCs) in rats.
Methods: the rat BMSCs was cultured and purified in vitro, and was induced by GSH. After the induction of 6h, 12h, and 24h, the quantitative PCR and Westblot were measured, and the neuron specific enolase (neuron specific enolase, NSE) and glial fibrillary acidic protein were measured and the protein levels were measured and the protein levels were measured. The expression.
Results: 6h, 12h, and 24h were induced to differentiate into neuron like cells and glial cell like cells. Real-time quantitative PCR and Westblot detection results showed that the expression of NSE and GFAP in BMSCs was significantly higher than that in the control group, and the 12h was the most obvious.
Conclusion: GSH can induce BMSCs to differentiate into neuron like cells and glial cell like cells, and increase the expression of NSE and GFAP mRNA and protein after induction.
Second basic fibroblast growth factors and epidermal growth factor induced gene expression profiles of rat bone marrow stromal cells to neuron like cells
Objective: To investigate the changes in gene expression profiles of rat bone marrow stromal stem cells (BMSCs) before and after differentiation into neuron like cells, especially the expression of genes related to neuron development.
Methods: the rat BMSCs was cultured and purified in vitro. Basic fibroblastgrowth factor (bFGF) and epidermal growth factor (epidermal growth factor, EGF) were used to induce them. The expression chips were detected by Affymetrix rats before and seventh days after induction, and the bioinformatics analysis technique was used. The changes of gene expression profiles before and after induction were compared.
Results: there were 216 different genes, of which 125 were up-regulated and 91 were down regulated. There were 13 genes closely related to the development of neurons, of which 7 were up and 6 were down.
Conclusion: the in vitro induced BMSCs has the potential to differentiate into neurons and can express some important genes related to the differentiation of neurons. These genes will be the focus of follow-up research.
【學位授予單位】：蘇州大學
【學位級別】：碩士
【學位授予年份】：2009
【分類號】：R329

【相似文獻】

相關期刊論文 前10條

1 孔進發(fā);毛玉文;;咐(口拜)醇酯TPA對慢性髓細胞白血病細胞的誘導分化作用[J];國際輸血及血液學雜志;1987年04期

2 林心建;黃自強;李常春;;洋地黃毒甙抗腫瘤作用及機理研究[J];福建醫(yī)科大學學報;1993年04期

3 耿雋超;朱恩良;單根法;鐘z，

本文編號：1979507