發(fā)布時(shí)間：2018-06-13 21:27

  本文選題：臍血移植 + 半相合��； 參考：《上海交通大學(xué)》2008年碩士論文

【摘要】： 目的:建立小鼠半相合臍血移植模型,探討多位點(diǎn)不相合臍血移植后造血及免疫重建特性。 方法:經(jīng)大劑量全身照射預(yù)處理BDF1小鼠后,經(jīng)尾靜脈輸注新生第一天DBA/2小鼠的外周全血,移植后動(dòng)態(tài)觀察受體鼠的造血重建及急性移植物抗宿主病(GVHD)發(fā)生情況,并用流式雙染色法進(jìn)一步檢測(cè)受鼠外周血、骨髓、脾和胸腺中粒系、淋巴系造血細(xì)胞的長(zhǎng)期植入水平。 結(jié)果:移植后小鼠的白細(xì)胞、血小板、血紅蛋白分別在第18、21、18天明顯回升,其中白細(xì)胞和血小板分別于第29天和36天恢復(fù)正常,而血紅蛋白至第50天仍為部分恢復(fù)。受體鼠90%長(zhǎng)期存活,且不發(fā)生明顯的GVHD。移植后第50天流式細(xì)胞儀檢測(cè)外周血中供鼠來源的造血細(xì)胞占總有核細(xì)胞的88.81%,其中含供者來源的粒細(xì)胞33.92%,T細(xì)胞16.83%;骨髓有核細(xì)胞以粒細(xì)胞和B細(xì)胞為主,分別占42.94%和37.24%;脾組織中仍殘留較多宿主T細(xì)胞,占總T細(xì)胞的42.2%;胸腺組織中T細(xì)胞達(dá)到完全嵌合;外周血、骨髓和脾組織CD11c+樹突狀細(xì)胞均達(dá)到或接近完全嵌合。 結(jié)論:單份新生小鼠外周血含有足夠的造血干細(xì)胞,可作為臍血移植的替代品,能重建半相合小鼠的造血及免疫系統(tǒng)而不引起明顯的GVHD,并可形成穩(wěn)定的以供者細(xì)胞為主的嵌合體。免疫器官和外周血的免疫細(xì)胞得到有效重建。
[Abstract]:Objective: to establish mouse haploidentical umbilical cord blood transplantation model and to study the hematopoietic and immune reconstitution characteristics after multi-locus incompatible cord blood transplantation. Methods: BDF1 mice were pretreated with high dose whole body irradiation. Peripheral whole blood of DBA / 2 mice on the first day of newborn was infused via tail vein. The hematopoietic reconstitution and GVHD of acute graft versus host disease were observed dynamically after transplantation. The long-term implantation level of hematopoietic cells in peripheral blood, bone marrow, spleen, thymus and lymphoid line of recipient rats was further detected by flow double staining. Results: the white blood cells, platelets and hemoglobin of the transplanted mice increased significantly on the 18th day after transplantation. The white blood cells and platelets returned to normal on the 29th and 36th day respectively, while the hemoglobin recovered partially on the 50th day. 90% of the recipient mice survived for a long time, and no obvious GVHDs occurred. On the 50th day after transplantation, the peripheral blood donor hematopoietic cells from mice accounted for 88.81% of the total nucleated cells, in which the donor granulocytes 33.92% T cells were 16.83% and the bone marrow nucleated cells were mainly granulocytes and B cells. The percentage of T cells in spleen tissues was 42.94% and 37.24%, respectively. There were still many host T cells in spleen tissues, which accounted for 42.2% of total T cells; T cells in thymus reached complete chimerism; CD11c dendritic cells in peripheral blood, bone marrow and spleen were all or close to complete chimerism. Conclusion: the single newborn mouse peripheral blood contains enough hematopoietic stem cells and can be used as a substitute for umbilical cord blood transplantation. It can reconstruct the hemopoiesis and immune system of haploidentical mice without causing obvious GVHD, and can form stable chimeric donor cells. Immune cells of immune organs and peripheral blood were reconstructed effectively.
【學(xué)位授予單位】：上海交通大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類號(hào)】：R-332;R457.7

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