發(fā)布時間：2018-07-08 21:17

  本文選題：契倫科夫光學成像 + 四環(huán)素��； 參考：《第三軍醫(yī)大學學報》2017年22期

【摘要】：目的探究四環(huán)素、熒光素鈉、異硫氰酸熒光素(fluorescein isothiocyanate,FITC)這3種臨床可用的熒光材料對契倫科夫光學信號增強的效果及最佳應用方案。方法將不同濃度的四環(huán)素溶液(100、50、10、5、1、0.5、0.1、0.05、0.01 mmol/L)、FITC溶液(5、4、3、2.5、1.5、1、0.5、0.25 mmol/L)和熒光素鈉溶液(100、50、10、5、2、1、0.2、0.1 mmol/L)分別與100μCi的~(18)F-FDG混合后進行契倫科夫光學成像(Cerenkov luminescence imaging,CLI),觀察契倫科夫光學信號的增強效果,并勾畫感興趣區(qū)域(region of interest,ROI),對光學信號強度進行定量分析,確定最佳信號增強效果的濃度,并對信號最強的板孔以20 nm為間隔進行500~800 nm范圍的光譜分離成像。同時進行在體實驗驗證熒光素鈉及FITC對契倫科夫光的增強效果。結果不同濃度的四環(huán)素溶液對光學信號無明顯影響。FITC及熒光素鈉均能顯著提高契倫科夫光學信號強度。當FITC及熒光素鈉濃度分別為1或2 mmol/L時產生的光學信號強度最強,為單獨的~(18)F-FDG的3.2倍及5倍。光譜分析表明2 mmol/L的熒光素鈉能將~(18)F-FDG產生的契倫科夫光(cerenkov luminescence,CL)光紅移至540 nm附近。荷瘤鼠在體實驗表明注射FITC或熒光素鈉后能使腫瘤部位的CL信號強度提高2倍或3.9倍。結論 FITC、熒光素鈉均能通過契倫科夫光的激發(fā)效應來增強契倫科夫光學信號強度,提高組織穿透力。
[Abstract]:Objective to investigate the effect of tetracycline, sodium fluorescein and fluorescein isothiocyanate on the enhancement of Cherenkov optical signal. Methods the different concentrations of tetracycline solution (100 ~ (50) C ~ (10) ~ (0.5) ~ (18) F-FDG) were mixed with 100 渭 ci ~ (18) F-FDG to observe the enhancement effect of Cerenkov luminescence imagingCLI, and the enhancement effect of Cerenkov luminescence imagingCLI was observed by observing the enhancement effect of Cerenkov optical signal, respectively, after mixing 100 渭 ci ~ (18) F-FDG with 100 ~ (50) 渭 ci ~ (18) F-FDG, respectively, and sodium fluorescein (100 ~ (50) 105210.22 ~ (0.1) mmol 路L ~ (-1) ~ (18) F ~ (18) F-FDG), respectively, in order to observe the enhancement of Cerenkov luminescence imagingCLI, and to observe the enhancement effect of Cerenkov luminescence imagingin CLI (100 ~ (18) F-FDG). The region of interest (region of) was drawn, the intensity of optical signal was quantitatively analyzed, the concentration of the best signal enhancement effect was determined, and the spectral separation imaging of the strongest plate hole was carried out in the range of 500-800 nm at an interval of 20 nm. At the same time, the enhancement effect of fluorescein sodium and FITC on Cherenkov light was tested in vivo. Results different concentrations of tetracycline solution had no significant effect on optical signal. FITC and sodium fluorescein could significantly improve the intensity of Cherenkov optical signal. When the concentration of FITC and sodium fluorescein was 1 or 2 mmol / L, the intensity of optical signal was the strongest, which was 3.2 and 5 times of that of ~ (18) F-FDG alone. Spectral analysis shows that 2 mmol / L sodium fluorescein can shift the cerenkov luminescence CL produced by ~ (18) F-FDG to about 540nm. In vivo experiments in tumor-bearing mice showed that FITC or sodium fluorescein could increase the signal intensity of CL in tumor site by 2 times or 3.9 times after injection of FITC or sodium fluorescein. Conclusion both FITC and sodium fluorescein can enhance the intensity of optical signal and enhance the penetration of tissue through the excitation effect of Cherenkov light.
【作者單位】： 武警總醫(yī)院消化內科;
【基金】：國家自然科學基金面上項目(81471700)~~
【分類號】：R817

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