乙型肝炎病毒核心基因的PCR-RELP圖案對(duì)中國(guó)肝細(xì)胞癌和肝硬化人群的臨床意義
發(fā)布時(shí)間：2025-05-15 05:04
簡(jiǎn)介：乙型肝炎是一種由乙型肝炎病毒所引起的疾病。它通過(guò)感染者的血液和體液進(jìn)行傳播。全世界約有4億人感染乙肝病毒，每年約150萬(wàn)人死亡。僅僅在中國(guó)就有1.3億人感染乙肝病毒。中國(guó)每年約有30萬(wàn)人死亡，其中包括18萬(wàn)肝細(xì)胞癌患者。乙肝病毒DNA包含4個(gè)開(kāi)放閱讀框：核心，表面，x和聚合酶。核心基因編碼乙型肝炎e抗原和乙型肝炎核心抗原。乙肝病毒核心基因突變和sNPs與慢性乙肝患者的肝炎活動(dòng)性有關(guān)。 目標(biāo)：1探清肝細(xì)胞癌和肝硬化患者乙肝病毒核心區(qū)域的PcR．RFLP模式。 2評(píng)價(jià)PCR-RFLP模式在肝細(xì)胞癌和肝硬化患者中的臨床意義。 材料與方法：從2010年1月1日到2010年11月7日共收集了320名肝細(xì)胞癌患者和130名肝硬化患者。根據(jù)統(tǒng)一的納入標(biāo)準(zhǔn)選擇患者�；颊叩哪挲g范圍從14歲到82歲。感染艾滋病病毒、丙肝病毒或有甲狀腺疾病的患者排除在外。某式PCR是用于擴(kuò)增HBV-DNA。有關(guān)乙肝病毒，肝細(xì)胞癌和肝硬化的實(shí)驗(yàn)分析分子生物學(xué)研究：HBV DNA，PcR-RFLP模式，測(cè)序 研究結(jié)果：有五個(gè)典型PcR-RFLP限制性酶裂解rsp509I模式（c，D，E...
【文章頁(yè)數(shù)】：43 頁(yè)
【學(xué)位級(jí)別】：碩士
【文章目錄】：
摘要
Abstract
1 Introduction
1.1 Chapter introduction
1.2 Hepatitis B
1.3 Hepatitis B virus structure, Mode of transmission and age, gender variation
1.4 Clinical course of hepatitis B infection
1.5 Epidemiology of Hepatitis B infection
1.6 Hepatitis B virus serotypes and genotypes
1.7 Hepatitis B virus infection and its role in Hepatocellular carcinoma and liver cirrhosis
1.8 Polymerase chain reaction, Restriction fragments Length Polymorphism (PCR- RFLP) of HBV core gene
2 Materials and methods
2.1 Introduction
2.2 Study Design
2.3 Study population
2.4 Clinical Data Collection
2.4.1 Demographic Details
2.4.2 Working Diagnosis
2.4.3 History Details
2.5 Sample collection and further procedures
2.5.1 Extraction of HBV DNA
2.5.2 Serology and other laboratory investigations
2.5.3 Real-time quantification of serum HBV-DNA
2.5.4 HBV Core Gene Specific PCR-RFLP
2.6 Data Arrangement and Measurements
2.6.1 Data Management
2.7 Ethical Issues
2.7.1 Ensuring confidentiality
2.7.2 Ensuring consent
2.7.3 Study Permissions
3 Results
3.1 HBV Core Gene Specific PCR-RFLP Patterns
3.2 Cleavage sites of Tsp509I on PCR-RFLP patterns
3.3 Serum HBV DNA, ALT and AFP levels in different RFLP patterns
3.4 Over all clinical data of HCC and liver cirrhosis patients
4 Discussion
5 Conclusion
References
Acknowledgement
本文編號(hào)：4046290
【文章頁(yè)數(shù)】：43 頁(yè)
【學(xué)位級(jí)別】：碩士
【文章目錄】：
摘要
Abstract
1 Introduction
1.1 Chapter introduction
1.2 Hepatitis B
1.3 Hepatitis B virus structure, Mode of transmission and age, gender variation
1.4 Clinical course of hepatitis B infection
1.5 Epidemiology of Hepatitis B infection
1.6 Hepatitis B virus serotypes and genotypes
1.7 Hepatitis B virus infection and its role in Hepatocellular carcinoma and liver cirrhosis
1.8 Polymerase chain reaction, Restriction fragments Length Polymorphism (PCR- RFLP) of HBV core gene
2 Materials and methods
2.1 Introduction
2.2 Study Design
2.3 Study population
2.4 Clinical Data Collection
2.4.1 Demographic Details
2.4.2 Working Diagnosis
2.4.3 History Details
2.5 Sample collection and further procedures
2.5.1 Extraction of HBV DNA
2.5.2 Serology and other laboratory investigations
2.5.3 Real-time quantification of serum HBV-DNA
2.5.4 HBV Core Gene Specific PCR-RFLP
2.6 Data Arrangement and Measurements
2.6.1 Data Management
2.7 Ethical Issues
2.7.1 Ensuring confidentiality
2.7.2 Ensuring consent
2.7.3 Study Permissions
3 Results
3.1 HBV Core Gene Specific PCR-RFLP Patterns
3.2 Cleavage sites of Tsp509I on PCR-RFLP patterns
3.3 Serum HBV DNA, ALT and AFP levels in different RFLP patterns
3.4 Over all clinical data of HCC and liver cirrhosis patients
4 Discussion
5 Conclusion
References
Acknowledgement
本文編號(hào)：4046290
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