發(fā)布時間：2018-07-11 21:44

  本文選題：金黃色葡萄球菌 + 生物被膜　； 參考：《中成藥》2015年08期

【摘要】：目的研究魚腥草素鈉(sodium houttuyfonate,SH)聯(lián)合阿奇霉素(azithromycin,AZM)對金黃色葡萄球菌生物被膜早期黏附的影響。方法微量倍比稀釋法測定魚腥草素鈉和阿奇霉素對金黃色葡萄球菌的最小抑菌濃度(minimum inhibitory concentration,MIC);連續(xù)稀釋法行活菌計數(shù),測定1 MIC魚腥草素鈉、1 MIC阿奇霉素單藥組和1/2MIC魚腥草素鈉+1/2MIC阿奇霉素聯(lián)合組對金黃色葡萄球菌生物被膜黏附的影響;結(jié)晶紫染色法評價不同濃度魚腥草素鈉和阿奇霉素單用及聯(lián)合組對金黃色葡萄球菌早期生物被膜生長量的影響;掃描電鏡(SEM)觀察各藥物組對金黃色葡萄球菌生物被膜形態(tài)的影響。結(jié)果魚腥草素鈉和阿奇霉素對金黃色葡萄球菌的MIC分別為64、0.25 mg/L。魚腥草素鈉聯(lián)合阿奇霉素聯(lián)合用藥組能增強阿奇霉素對生物被膜早期黏附的清除作用,尤以2MIC藥物濃度聯(lián)合組作用最強(與陰性對照組比較P0.001)。電鏡下觀察細菌數(shù)及形態(tài)也驗證了上述結(jié)果。結(jié)論魚腥草素鈉、阿奇霉素可減少金黃色葡萄球菌的早期黏附,抑制生物被膜的形成,聯(lián)合用藥后對金黃色葡萄球菌生物被膜的抑制具有協(xié)同作用,預期兩藥聯(lián)合使用可以提高生物被膜相關(guān)感染的臨床療效。
[Abstract]:Objective to study the effect of sodium houttuyfonate SH combined with azithromycin on the early adhesion of Staphylococcus aureus biofilm. Methods the minimal inhibitory concentration of sodium houttuyfonate and azithromycin on Staphylococcus aureus was determined by microdilution method, and the live bacteria count was determined by continuous dilution method. The effects of 1 MIC houttuynin sodium and 1 MIC azithromycin group and 1 / 2 MIC sodium houttuynin sodium 1 / 2 MIC azithromycin group on the adhesion of staphylococcus aureus biofilm were determined. The effects of sodium houttuyfonate and azithromycin on the growth of early biofilm of Staphylococcus aureus were evaluated by crystal violet staining. Scanning electron microscopy (SEM) was used to observe the effect of each drug group on the morphology of staphylococcus aureus biofilm. Results the MIC of houttuynin sodium and azithromycin against Staphylococcus aureus were 640.25mg / L respectively. Sodium houttuyfonate combined with azithromycin could enhance the scavenging effect of azithromycin on the early adhesion of biofilm, especially in the 2MIC drug concentration group (P0.001 compared with the negative control group). The number and morphology of bacteria under electron microscope also confirmed the above results. Conclusion Sodium houttuyfonate and azithromycin can reduce the early adhesion of Staphylococcus aureus and inhibit the formation of biofilm. The combined use of Houttuynin and azithromycin has synergistic effect on the inhibition of biofilm of Staphylococcus aureus. It is expected that the combined use of the two drugs can improve the clinical efficacy of biofilm associated infection.
【作者單位】： 安徽中醫(yī)藥大學;
【基金】：國家自然科學基金項目(81173629)
【分類號】：R96
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本文編號：2116423