發(fā)布時(shí)間：2018-07-12 20:03

  本文選題：尿石素A + 巨噬細(xì)胞極化　； 參考：《食品科學(xué)》2017年13期

【摘要】：旨在探究尿石素A對巨噬細(xì)胞極化及對巨噬-泡沫細(xì)胞形成的影響及相關(guān)的分子機(jī)制。結(jié)果發(fā)現(xiàn),尿石素A通過調(diào)控不同標(biāo)志基因的表達(dá),不僅能夠抑制RAW264.7小鼠巨噬細(xì)胞向促炎的M1型巨噬細(xì)胞極化,還能促進(jìn)自然狀態(tài)巨噬細(xì)胞向M2型巨噬細(xì)胞極化;油紅O染色發(fā)現(xiàn)尿石素A能夠顯著抑制巨噬-泡沫細(xì)胞形成,實(shí)時(shí)熒光定量聚合酶鏈?zhǔn)椒磻?yīng)檢測說明尿石素A能夠抑制膽固醇合成基因羥甲基戊二酸單酰輔酶A還原酶和脂肪酸合成酶基因的轉(zhuǎn)錄;此外,尿石素A顯著上調(diào)三磷酸腺苷結(jié)合盒轉(zhuǎn)運(yùn)蛋白A1和G1基因的表達(dá),該兩個(gè)基因表達(dá)與促進(jìn)了膽固醇的排出相關(guān)。本研究證明了尿石素A對巨噬細(xì)胞極化具有調(diào)控作用,同時(shí)尿石素A能夠抑制巨噬-泡沫細(xì)胞形成和膽固醇合成。這些結(jié)果揭示了尿石素A具有潛在的抗動(dòng)脈粥樣硬化的作用,為之后深入研究提供了理論參考。
[Abstract]:The aim of this study was to investigate the effects of urolitol A on the polarization of macrophages and the formation of macrophage-foam cells and the related molecular mechanisms. The results showed that urolitol A could not only inhibit the polarization of macrophages from RAW264.7 mice to type M1 macrophages, but also promote the polarization of natural macrophages to M2 type macrophages by regulating the expression of different marker genes. Oil red O staining showed that urostone A could significantly inhibit the formation of macrophages and foam cells. Real-time fluorescence quantitative polymerase chain reaction showed that urostone A could inhibit the transcription of the cholesterol synthase gene hydroxymethyl glutaryl coA reductase and fatty acid synthase gene. The expression of adenosine triphosphate binding cassette transporter A1 and G1 genes was significantly up-regulated by urolithiasis A, which was related to the promotion of cholesterol excretion. This study demonstrated that urolitol A can regulate the polarization of macrophages, and urolide A can inhibit the formation of macrophage foam cells and cholesterol synthesis. These results suggest that urolitol A has a potential anti-atherosclerosis effect, which provides a theoretical reference for further research.
【作者單位】： 西北農(nóng)林科技大學(xué)食品科學(xué)與工程學(xué)院;中國農(nóng)業(yè)大學(xué)食品科學(xué)與營養(yǎng)工程學(xué)院北京食品營養(yǎng)與人類健康高精尖創(chuàng)新中心;定興縣醫(yī)院;西安交通大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：教育部“新世紀(jì)優(yōu)秀人才支持計(jì)劃”項(xiàng)目(NCET-13-0488) “十二五”國家科技支撐計(jì)劃項(xiàng)目(2015BAD16B08) 北京市食品營養(yǎng)與人類健康高精尖創(chuàng)新中心項(xiàng)目(201502910110942) 中央高校基本科研業(yè)務(wù)費(fèi)專項(xiàng)資金項(xiàng)目(2452017146)
【分類號】：R151

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王帥;劉明s，

本文編號：2118317