發(fā)布時(shí)間：2018-02-02 02:44

  本文關(guān)鍵詞： 肝癌 癌旁 黑色素瘤缺乏因子2 半胱天冬酶-1 白介素-1β　出處：《山東大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：[背景／目的]慢性乙型肝炎相關(guān)性肝癌的發(fā)病機(jī)制目前尚不明確,目前認(rèn)為其發(fā)病主要與乙肝病毒與宿主基因整合、慢性肝臟炎癥有關(guān)。近年來(lái),發(fā)現(xiàn)有很多細(xì)胞因子參與了慢性乙型肝炎的炎癥反應(yīng)過(guò)程,其中AIM2是目前研究的熱點(diǎn)之一。AIM2(黑色素瘤缺乏因子2)是一種主要定位于細(xì)胞質(zhì)的蛋白質(zhì),它可以與細(xì)胞質(zhì)雙鏈DNA結(jié)合,誘導(dǎo)ASC(凋亡相關(guān)的斑點(diǎn)樣蛋白)炎性復(fù)合體的形成,激活caspase-1(半胱天冬酶-1),促進(jìn)IL-1β(白細(xì)胞介素-1β)的釋放,誘導(dǎo)機(jī)體固有免疫反應(yīng),抵御病毒、細(xì)菌的感染。本研究旨在探討AIM2在慢性乙型肝炎相關(guān)性肝癌的發(fā)生和發(fā)展過(guò)程中的作用。[方法]50例肝癌患者手術(shù)切除的肝癌以及癌旁組織,肝癌的診斷符合病理學(xué)診斷標(biāo)準(zhǔn)。所有患者HBsAg陽(yáng)性且均未見(jiàn)遠(yuǎn)隔轉(zhuǎn)移灶,在手術(shù)前均未進(jìn)行過(guò)化療或放療,亦未進(jìn)行過(guò)免疫抑制劑治療。排除合并其他病毒感染所致的肝炎。用免疫組織化學(xué)方法分別測(cè)定肝癌和癌旁組織中AIM2、caspase-1、IL-1β的表達(dá)。組間比較應(yīng)用卡方檢驗(yàn)和秩和檢驗(yàn),相關(guān)性分析應(yīng)用spearman相關(guān)分析。[結(jié)果]AIM2在肝癌組織中的陽(yáng)性表達(dá)率高于癌旁組織(X2=8.274,P0.05),肝癌組織中AIM2的表達(dá)強(qiáng)度明顯高于癌旁組織(Z。=3.243,P0.05)。e抗原表達(dá)陽(yáng)性者AIM2表達(dá)強(qiáng)度均高于陰性者(肝癌：Z。=2.620,P0.05；癌旁：Z。=2.367,P0.05)。高病毒載量患者(HBV-DNAS≥1×105copies/ml)中AIM2的表達(dá)顯著高于低病毒載量患者(HBV-DNA1×105copies/ml)(肝癌組織：Zc=3.242,P0.05；癌旁組織：Z。=3.379,P0.05)。肝癌組織中AIM2的表達(dá)與ALT、AST、AFP水平的高低有關(guān)(rs=0.333,0.548,0.336, P0.05)。Caspase-1的表達(dá)與AIM2的表達(dá)呈正相關(guān)(肝癌：rs=O.903,p0.01；癌旁：rs=O.910,p0.01)；IL-1 β的表達(dá)與AIM2的表達(dá)呈正相關(guān)(肝癌rs=0.905,p0.01；癌旁：rs=0.841,p0.01)。[結(jié)論]1.AIM2在肝癌組織中的陽(yáng)性表達(dá)主要位于細(xì)胞質(zhì)中,肝癌組織中AIM2的表達(dá)程度高于癌旁組織。2.HBV病毒載量高的患者AIM2表達(dá)程度較高, e抗原陽(yáng)性的患者AIM2表達(dá)程度較高, AIM2表達(dá)程度與AST、ALT水平呈正相關(guān)性,AIM2的表達(dá)與患者的年齡、性別、PLT以及白蛋白水平無(wú)關(guān)。3.AIM2的表達(dá)程度與AFP的水平呈正相關(guān)性,而與肝癌分級(jí)無(wú)關(guān)。
[Abstract]:[Background / objective: the pathogenesis of chronic hepatitis B associated liver cancer is still unclear. It is believed that the pathogenesis is mainly related to the integration of hepatitis B virus with host genes and chronic liver inflammation. Many cytokines were found to be involved in the inflammatory response of chronic hepatitis B. AIM2 (melanoma deficiency factor 2) is a major cytoplasmic protein, which can bind to cytoplasmic double-stranded DNA. Asc (apoptosis-related dot-like protein) inflammatory complex was induced, caspase-1 (caspase-1) was activated and IL-1 尾 (interleukin-1 尾) was released. The purpose of this study was to investigate the role of AIM2 in the occurrence and development of chronic hepatitis B related liver cancer. [Methods] the diagnosis of liver cancer in 50 patients with liver cancer was in accordance with the pathological diagnostic criteria. HBsAg positive and no distant metastasis was found in all the patients. No chemotherapy or radiotherapy or immunosuppressive therapy was performed before the operation. Hepatitis caused by other virus infection was excluded. AIM2 in liver cancer and adjacent tissues were determined by immunohistochemical method. Expression of caspase-1 and IL-1 尾. Chi-square test and rank sum test were used in the comparison between groups. Correlation analysis was performed with spearman correlation analysis. [Results the positive expression rate of AIM2 in HCC tissues was higher than that in paracancerous tissues (P 0.05). The expression of AIM2 in HCC tissues was significantly higher than that in adjacent tissues. The expression intensity of AIM2 was higher in the positive expression of P0.05n.e antigen than that in the negative group (P0.05). Next to cancer: Z. 2.367. The expression of AIM2 in patients with high viral load was significantly higher than that in patients with low viral load (HBV-DNA S 鈮，

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