發(fā)布時間：2018-08-26 13:17

【摘要】：目的近年來的研究表明,人參皂苷Rd(ginsenoside Rd,G-Rd)具有神經(jīng)保護(hù)、抗炎等作用,但目前尚不清楚人參皂苷Rd在腸道上皮細(xì)胞中是否會影響炎癥反應(yīng)。本課題旨在探討人參皂苷Rd對脂多糖(lipopolysaccharide,LPS)誘導(dǎo)的人結(jié)腸癌細(xì)胞系HT-29細(xì)胞炎癥反應(yīng)的影響及作用機(jī)制。方法利用RT-qPCR(Real time-quantitative polymerase chain reaction)檢測G-Rd對LPS刺激的HT-29細(xì)胞炎癥因子IL-8的mRNA表達(dá)水平的影響;利用ELISA技術(shù)檢測G-Rd對LPS刺激的HT-29細(xì)胞分泌的炎癥因子IL-8蛋白表達(dá)水平的影響;利用免疫印跡法(Western blotting)、免疫熒光技術(shù)、EMSA檢測G-Rd對LPS誘導(dǎo)的HT-29細(xì)胞NF-κB信號通路的影響。結(jié)果1.RT-qPCR及ELISA實驗表明:G-Rd能從mRNA水平及蛋白質(zhì)水平上抑制由LPS誘導(dǎo)的HT-29細(xì)胞中IL-8的表達(dá),且呈現(xiàn)劑量依賴關(guān)系。2.免疫熒光、免疫印跡及EMSA實驗表明:G-Rd可下調(diào)LPS誘導(dǎo)的NF-κB信號通路的活化水平。結(jié)論G-Rd能通過抑制LPS刺激HT-29細(xì)胞分泌炎癥因子IL-8而發(fā)揮抗炎作用,其機(jī)制可能與抑制NF-κB信號通路活化有關(guān)。
[Abstract]:Objective recent studies have shown that ginsenoside Rd (ginsenoside Rd,G-Rd has neuroprotective and anti-inflammatory effects, but it is not clear whether ginsenoside Rd affects inflammation in intestinal epithelial cells. The aim of this study was to investigate the effect and mechanism of ginsenoside Rd on lipopolysaccharide (lipopolysaccharide,LPS) -induced inflammatory response of human colon cancer cell line HT-29. Methods RT-qPCR (Real time-quantitative polymerase chain reaction) was used to detect the effect of G-Rd on the mRNA expression of IL-8 in HT-29 cells stimulated by LPS, and ELISA technique was used to detect the effect of G-Rd on the expression of IL-8 protein, which was secreted by LPS stimulated HT-29 cells. The effect of G-Rd on NF- 魏 B signaling pathway in HT-29 cells induced by LPS was detected by immunoblotting (Western blotting), immunofluorescence technique. Results 1.RT-qPCR and ELISA experiments showed that G Rd could inhibit the expression of IL-8 in HT-29 cells induced by LPS at the level of mRNA and protein in a dose-dependent manner. Immunofluorescence, immunoblotting and EMSA assay showed that the activation level of NF- 魏 B signaling pathway induced by LPS could be down-regulated by 1: G Rd. Conclusion G-Rd can play an anti-inflammatory effect by inhibiting the secretion of inflammatory factor IL-8 by HT-29 cells stimulated by LPS. The mechanism may be related to the inhibition of activation of NF- 魏 B signaling pathway.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R735.35

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉偉;繆建春;;人參皂苷CK的抗炎及神經(jīng)保護(hù)作用研究[J];中國醫(yī)藥導(dǎo)報;2013年21期

2 苗雪;官杰;;IL-8的研究進(jìn)展[J];齊齊哈爾醫(yī)學(xué)院學(xué)報;2011年22期

3 張琛;趙鋼;;人參皂苷Rd的藥理作用研究進(jìn)展[J];中國新藥雜志;2011年11期

4 趙保勝;桂海水;徐暾海;;人參皂苷Rg1抗炎作用實驗研究[J];人參研究;2010年04期

5 于振海;陳立東;王志強(qiáng);徐寧;;Toll樣受體4、NF-κB在潰瘍性結(jié)腸炎中的表達(dá)[J];細(xì)胞與分子免疫學(xué)雜志;2010年07期

6 呂振超;郭艷幸;曹向陽;陳武林;丁幸坡;邊靖;;人參皂苷Rg1、Rb1對體外培養(yǎng)膝軟骨細(xì)胞凋亡的影響[J];世界中西醫(yī)結(jié)合雜志;2010年01期

7 何姜;劉禮斌;;轉(zhuǎn)錄因子NF-κB生物特性及應(yīng)用前景[J];醫(yī)學(xué)綜述;2009年14期

8 林力;劉建勛;張穎;段昌令;林成仁;;LC-MS/MS法測定大鼠口服人參皂苷Rg_1,Re,Rb_1和Rd的藥動學(xué)[J];中國藥學(xué)雜志;2009年05期

9 Epameinondas V Tsianos;Konstantinos Katsanos;;Do we really understand what the immunological disturbances in inflammatory bowel disease mean?[J];World Journal of Gastroenterology;2009年05期

10 王為;周國華;;細(xì)胞因子與炎癥性腸病[J];臨床軍醫(yī)雜志;2007年03期

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