本文關(guān)鍵詞：風(fēng)向因素對轉(zhuǎn)基因抗蟲棉花基因漂移效率的影響，由筆耕文化傳播整理發(fā)布。

風(fēng)向因素對轉(zhuǎn)基因抗蟲棉花基因漂移效率的影響

The influence of wind direction on pollen-mediated gene flow in transgenic insect-resistant cotton

[1] [2] [3] [4] [5]

ZHU Jialin, HE Juan, NIU Jianqun, ZHANG Qingwen, LIU Xiaoxia Department of Entomology, China Agricultural University, Beijing 100193, China

中國農(nóng)業(yè)大學(xué)昆蟲學(xué)系,北京100193

文章摘要：在轉(zhuǎn)基因作物獲準(zhǔn)進(jìn)行環(huán)境釋放并實(shí)行大面積商品化推廣的同時(shí)，基因漂移所引起的生態(tài)環(huán)境安全問題不容忽視。以含有雙價(jià)抗蟲基因（Bt/CpTI）的轉(zhuǎn)基因棉花SGK321為花粉供體材料，以常規(guī)非轉(zhuǎn)基因棉花品種石遠(yuǎn)321、中棉35、吉扎1號為花粉受體材料，在溫室中人工創(chuàng)造定向風(fēng)和非定向風(fēng)條件，，應(yīng)用PCR與蛋白檢測相結(jié)合的方法，檢測外源基因發(fā)生基因漂移的效率。結(jié)果表明：隨著與轉(zhuǎn)基因棉花SGK321距離的增加，外源基因轉(zhuǎn)移至非轉(zhuǎn)基因棉花的基因漂移頻率呈現(xiàn)波動性變化。在定向風(fēng)處理中，基因漂移頻率在距離轉(zhuǎn)基因棉花6.4m處達(dá)到峰值33.33%，在測定范圍內(nèi)基因漂移最遠(yuǎn)距離為25.6m；而在非定向風(fēng)處理中，基因漂移頻率在距離轉(zhuǎn)基因棉花12.8m處達(dá)到峰值36.67%，在測定范圍內(nèi)基因漂移最遠(yuǎn)距離為36m。非定向風(fēng)可顯著提高轉(zhuǎn)移至海島棉吉扎1號的基因漂移頻率。外源基因從SGK321轉(zhuǎn)移至其非轉(zhuǎn)基因親本石遠(yuǎn)321的基因漂移頻率顯著高于轉(zhuǎn)移至陸地棉中棉35和海島棉吉扎1號的漂移頻率。為轉(zhuǎn)基因棉花的生態(tài)安全性分析提供一定的理論參考價(jià)值。

Abstr：With the extensive environment release and large scale cultivation of genetically modified （GM） crops, the ecological environment security problems, caused by transgene escape from GM crop to its non-GM counterpart and wild relatives, have appeared gradually. During the early years of breeding and seed increase, there are some uncertainty among regulators about containment measures needed to prevent the movement of regulated GM traits into adjacent fields and possibly into the human or animal food chain. Cotton, one of the wind-pollinated and highly outcrossing cash crops, is planted on millions of hectares annually and is the third most abundant GM crop.This study investigated the occurrence of gene flow from transgenic cotton to non-transgenic cottons under closed conditions using an insect-resistant gene as a tracer marker. The selectable marker used in this study was the synthetic GFM CryⅠA（C）gene which encoded Bt insecticidal protein with resistance to cotton bollworm. In order to determine the impacts of wind direction on pollen-mediated gene flow （PGF）, two greenhouse experiments under different wind conditions （directed and indirected） were establishied. The growth area for each treatment was 480m2, and the row and plant spacing was 80cm×30cm. To observe the frequency and distance of gene flow, the transgenic cotton, SGK321 （Gossypium hirsutum L.）, was used as a pollen donor, and conventional varieties Shiyuan321 （Gossypium hirsutum L.）, Zhongmian35 （Gossypium hirsutum（ L.） and Jizha1 （Gossypium barbadense L.） were applied as pollen recipients, separately. Following natural pollination under the wind conditions, the seeds were collected from each conventional varieties at varying distances and sown in pot cul

文章關(guān)鍵詞：

Keyword：:transgenic cotton Bt gene transgene escape qualitative PCR biosafety

課題項(xiàng)目：轉(zhuǎn)基因生物新品種培育科技重大專項(xiàng)（2011ZX0811-002）

作者信息：會員可見

  本文關(guān)鍵詞：風(fēng)向因素對轉(zhuǎn)基因抗蟲棉花基因漂移效率的影響，由筆耕文化傳播整理發(fā)布。