本文關(guān)鍵詞：不同蟲壓下轉(zhuǎn)Bt基因水稻與非轉(zhuǎn)基因水稻生態(tài)適合度差異，由筆耕文化傳播整理發(fā)布。

不同蟲壓下轉(zhuǎn)Bt基因水稻與非轉(zhuǎn)基因水稻生態(tài)適合度差異

Differences in Ecological Fitness Between Bt Transgenic Rice and Regular Rice Under Different Insect-infestation Pressures

[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12]

ZHANG Fuli,LIU Yong,TONG Hongjin,YIN Quan,TAO Li,WANG Dong,ZHOU Xiquan,CHANG Lijuan,SONG Jun,LIU Wenjuan,LEI Shaorong, GUO Ling＇an（1Analysis and Determination Center,Sichuan Academy

[1]四川省農(nóng)業(yè)科學(xué)院分析測試中心,成都610066; [2]四川省農(nóng)業(yè)科學(xué)院植物保護研究所,成都610066; [3]四川省環(huán)境保護科學(xué)研究院,成都610064

文章摘要：為了解轉(zhuǎn)基因水稻的基因擴散效率和潛在生態(tài)風(fēng)險,以轉(zhuǎn)Bt抗蟲水稻Bt63、R1、R2和非轉(zhuǎn)基因常規(guī)水稻Ⅱ優(yōu)838為試材,采用高、低兩個不同蟲害脅迫水平和轉(zhuǎn)基因與非轉(zhuǎn)基因水稻相間種植方式,通過觀察水稻植株營養(yǎng)生長、結(jié)實以及對螟蟲危害的抗性表現(xiàn)等差異,研究比較了Bt外源基因插入后對水稻植株適合度的影響.結(jié)果表明：在低蟲害脅迫條件下,轉(zhuǎn)Bt基因水稻在植株分蘗數(shù)、生物量鮮重等營養(yǎng)生長指標(biāo)方面與非轉(zhuǎn)基因?qū)φ掌废甸g無明顯差異,但株高、穗長、穗重等指標(biāo)不及對照,且R2和Bt63與對照間差異顯著;在高蟲害脅迫條件下,3個轉(zhuǎn)Bt基因水稻品系的分蘗數(shù)、穗長、穗重等指標(biāo)明顯高于對照.而不同轉(zhuǎn)基因品系株高適合度效應(yīng)不同,這可能與受體本身的特性相關(guān).3種轉(zhuǎn)基因水稻的單株結(jié)實粒數(shù)、千粒重與對照在兩種蟲害脅迫條件下均無顯著性差異,Bt基因?qū)κ荏w植株的結(jié)實影響不明顯.在高蟲害脅迫條件下,3種轉(zhuǎn)Bt基因水稻的抗蟲能力均顯著優(yōu)于非轉(zhuǎn)基因水稻,表明Bt基因?qū)κ荏w植株的抗蟲性影響顯著.同時,本研究結(jié)果還表明轉(zhuǎn)Bt基因水稻的適合度代價較小,預(yù)示了抗蟲轉(zhuǎn)基因水稻外源Bt基因在一定環(huán)境條件下具有逃逸的可能,但這種風(fēng)險比較小.

Abstr：For understanding the possibility and efficiency of foreign gene spread in nature and some potential ecological risk of transgenic rice,a experiment was carried out to investigate the effect of foreign Bt gene insertion on the ecological fitness of the transgenic rice plant.Three Bt transgenic rice cultivars,＇Bt63＇,＇R1＇ and ＇R2＇,and a non-transgenic rice ＇Ⅱ-838＇ were used as materials.The insect-resistant transgenic and the non-transgenic rice plants were inter-cropped pair-wisely.Two levels of rice stem borer-infestation were set up.The vegetative growth,seed-setting and the resistance to rice stem borers were compared between transgenic and non-transgenic lines.The results showed that both number of tillers and fresh weight of the transgenic rice plant were no differences compared with the control under low pest-infestation pressure,but the plant height,spike length and weight were all lower than those of the non-transgenic rice plant,and ＇Bt63＇ and ＇R2＇ were significantly different compared with the control.On the contrary,the number of tillers,spike weight and the length of the transgenic rice plant under high pest-infestation level were significantly higher than those of the control.The plant height was different amongst the transgenic lines,which was presumably because of the receptive cultivars＇ traits.Both the number of seeds and thousand-grain weight were no differences between the transgenic and non-transgenic rice lines under two different insect-infestation pressures,suggesting that the effect of foreign Bt gene on seed setting was not significant.In contrast to the non-transgenic rice,three Bt transgenic rice lines displayed a higher resistance to rice stem borer.The fitness of the Bt transgenic rice was distinctly

文章關(guān)鍵詞：

Keyword：:ecological fitness insect-resistance transgenic rice insect-infestation pressure gene spread ecological risk

課題項目：農(nóng)業(yè)部轉(zhuǎn)基因重大專項（No.2011ZX08011-006）資助

作者信息：會員可見

  本文關(guān)鍵詞：不同蟲壓下轉(zhuǎn)Bt基因水稻與非轉(zhuǎn)基因水稻生態(tài)適合度差異，由筆耕文化傳播整理發(fā)布。