發(fā)布時間：2018-01-19 08:48

  本文關(guān)鍵詞： 急性白血病 自噬 免疫組織化學染色 Atg5 Atg7 蛋白　出處：《河北醫(yī)科大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:急性白血病(acute leukemia,AL)是一類常見的造血干細胞惡性增殖性疾病,目前認為自噬調(diào)控失調(diào)與腫瘤發(fā)生密切相關(guān)。自噬是真核細胞在自噬相關(guān)基因(autophagy related gene,Atg)的調(diào)控下利用溶酶體降解受損細胞器和大分子物質(zhì)的過程,在維持細胞穩(wěn)定方面起重要作用,是細胞適應內(nèi)外環(huán)境所需的一種基本調(diào)節(jié)方式。采用免疫組織化學染色法對兒童急性白血病骨髓細胞中自噬相關(guān)基因Atg5、Atg7蛋白表達水平進行檢測,探討這兩種因子活性與兒童急性白血病發(fā)生、發(fā)展的關(guān)系。方法1研究對象:實驗組隨機選取自2014年1月至2016年6月河北醫(yī)科大學第二醫(yī)院兒科住院的初診未治急性白血病患兒50例,其中急性淋巴細胞白血病組30例,急性髓細胞白血病組20例,對照組選取同期與實驗組年齡性別相匹配的非惡性血液病(包括免疫性血小板減少癥、營養(yǎng)性貧血)患兒20例。2標本采集和處理:分別抽取實驗組及對照組新鮮骨髓標本2ml,自然凝固后經(jīng)4%多聚甲醛固定,常規(guī)石蠟包埋、切片。3實驗方法:采用免疫組織化學染色法檢測兒童急性淋巴細胞白血病、急性髓細胞白血病及對照組骨髓細胞中Atg5、Atg7蛋白的表達水平。4統(tǒng)計學分析:應用SPSS13.0統(tǒng)計分析軟件進行統(tǒng)計學分析,Atg5、Atg7蛋白在各組標本中表達強度采用x2檢驗或Fisher確切概率法,P0.05有統(tǒng)計學意義;Atg5、Atg7蛋白的相關(guān)性使用Spearman相關(guān)性分析進行檢驗,P0.01有統(tǒng)計學意義。結(jié)果1 Atg5蛋白的表達情況1.1 Atg5在急性白血病中陽性率(82.0%)高于對照組陽性率(40.0%),兩者之間表達差異有統(tǒng)計學意義(x2=12.0,P0.05)。1.2 Atg5在急性淋巴細胞白血病中陽性率為83.3%,在急性髓細胞白血病中陽性率80.0%,分別同對照組進行比較,各組差異有統(tǒng)計學意義(x2=12.063,P0.05)。1.3 Atg5在急性淋巴細胞白血病同急性髓細胞白血病比較,兩者表達差異無統(tǒng)計學意義(x2=0.090,P0.0125)。1.4 Atg5在急性淋巴細胞白血病同對照組比較,兩者表達差異有統(tǒng)計學意義(x2=10.042,P0.0125)。1.5 Atg5在急性髓細胞白血病同對照組比較,兩者表達差異有統(tǒng)計學意義(x2=6.667,P0.0125)。2 Atg7蛋白的表達情況2.1 Atg7在急性白血病中陽性率54.0%,在對照組陽性率30.0%,兩者表達差異無統(tǒng)計學意義(x2=3.302,P0.05)。2.2 Atg7在急性淋巴細胞白血病中陽性率43.3%,在急性髓細胞白血病中陽性率70.0%,分別同對照組進行比較,各組差異有統(tǒng)計學意義(x2=6.7278,P0.05)。2.3 Atg7在急性淋巴細胞白血病同急性髓細胞白血病比較,兩者間表達水平無明顯差異(x2=3.435,P0.0125)。2.4 Atg7在急性淋巴細胞白血病同對照組比較,兩者間表達水平無明顯差異(x2=0.905,P0.0125)。2.5 Atg7在急性髓細胞白血病同對照組比較,兩者表達差異有統(tǒng)計學意義(x2=6.400,P0.0125)。3利用Spearman秩相關(guān)性檢驗比較Atg5及Atg7蛋白表達是否相關(guān),求得r=0.333,P0.01,兩者在急性白血病中的表達無明顯相關(guān)性。結(jié)論1 Atg5蛋白在急性淋巴細胞白血病及急性髓細胞白血病中表達上調(diào),Atg5可能參與急性白血病的發(fā)生發(fā)展;Atg7蛋白在急性髓細胞白血病中表達上調(diào),自噬活性增高,提示Atg7可能與急性髓細胞白血病的發(fā)生、發(fā)展有關(guān)。2實驗發(fā)現(xiàn)Atg5、Atg7在急性白血病及非惡性血液病中均有表達,但其表達水平受疾病種類、分型的影響。3 Atg5、Atg7雖然都參與自噬體形成過程,但是兩者在急性白血病中的表達無明顯相關(guān)性。
[Abstract]:Objective: acute leukemia (acute leukemia AL) is a kind of common malignant hematopoietic stem cell proliferative diseases, the autophagy dysfunction is related to tumorigenesis. Autophagy is eukaryotic cells in autophagy related genes (autophagy related, gene, Atg) under the regulation and process of damaged organelles and macromolecules with the lysosomal degradation plays an important role in maintaining cell stability, is a basic regulation that cells adapt to internal and external environment. Immunohistochemical staining of bone marrow cells autophagy related gene Atg5 in children with acute leukemia, the expression level of Atg7 protein was detected on the two factor activity and childhood acute leukemia occurrence, development the relationship between the 1 subjects. Methods: in the experimental group were randomly selected from newly diagnosed pediatric hospital of Hebei Medical University second hospital from January 2014 to June 2016 in untreated acute leukemia 50 cases of children with acute lymphoblastic leukemia group 30 cases, 20 cases of acute myeloid leukemia group, control group and experimental group were selected to match the age and sex of non malignant hematological diseases (including immune thrombocytopenia, anaemia) in 20 children with.2 were collected and processed separately from: experimental group and control group fresh bone marrow specimens of 2ml, coagulation after 4% paraformaldehyde, embedded in paraffin, sliced.3 experimental methods: immunohistochemistry staining in children with acute lymphoblastic leukemia, acute myeloid leukemia and control group Atg5 in bone marrow cells, the expression level of.4 protein was Atg7 analysis: using SPSS13.0 statistical analysis software statistical analysis, Atg5, Atg7 protein expression in the specimens in strength using x2 test or Fisher exact test, P0.05 had statistical significance; Atg5, using the correlation of protein Atg7 Spearman correlation analysis, P0.01 was statistically significant. The expression of Atg5 1 protein 1.1 Atg5 in acute leukemia positive rate (82%) higher than that of the control group (positive rate 40%), there is a significant difference between the two (x2=12.0, P0.05).1.2 Atg5 in acute lymphoblastic leukemia positive rate was 83.3% in acute myeloid leukemia with positive rate of 80%, respectively, compared with the control group, the difference was statistically significant (x2=12.063, P0.05).1.3 Atg5 in acute lymphoblastic leukemia with acute myelogenous leukemia compared the expression difference was not statistically significant (x2=0.090, P0.0125).1.4 Atg5 in acute lymphoblastic leukemia compared with the control group, the expression of statistically significant difference (x2=10.042, P0.0125).1.5 Atg5 in acute myeloid leukemia compared with the control group, there were statistical significance of difference (x2=6.66 7, P0.0125) expression of.2 Atg7 protein 2.1 Atg7 in acute leukemia and the positive rate was 54%, the positive rate was 30% in the control group, the expression of the difference was not statistically significant (x2=3.302, P0.05).2.2 Atg7 in acute lymphoblastic leukemia with positive rate of 43.3%, in acute myeloid leukemia with positive rate of 70%, respectively, with the control groups were compared, the difference was statistically significant (x2=6.7278, P0.05).2.3 Atg7 in acute lymphoblastic leukemia compared with acute myeloid leukemia, there was no difference between the expression level of.2.4 Atg7 (x2=3.435, P0.0125) in acute lymphoblastic leukemia compared with the control group, no significant difference between the two levels (x2=0.905, P0.0125).2.5 Atg7 in acute myelogenous leukemia compared with the control group, the expression difference was statistically significant (x2=6.400, P0.0125).3 by the Spearman rank correlation test between the Atg5 and Atg7 protein expression As if, obtained r=0.333, P0.01, there was no significant correlation between their expression in acute leukemia. Conclusion the expression of Atg5 protein in 1 patients with acute lymphoblastic leukemia and acute myeloid leukemia up-regulated Atg5 may be involved in the development of acute leukemia; expression of Atg7 protein in acute myeloid leukemia up-regulated autophagy activity was increased, suggesting that Atg7 may be associated with acute myeloid leukemia, the development of the.2 experiments found that Atg5, the expression of Atg7 in acute leukemia and non malignant hematological diseases were, but its expression level by disease type,.3 Atg5 type Atg7 effects, although in autophagosome formation, but their expression in acute leukemia has no obvious correlation.

【學位授予單位】：河北醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R733.71

【參考文獻】

相關(guān)期刊論文 前9條

1 米萱;孫峰;仇利紅;石定;余州;楊力;;自噬相關(guān)蛋白Atg5和LC3在嬰幼兒血管瘤中的表達[J];中國美容整形外科雜志;2016年06期

2 周藝;聶玉強;林泳;杜艷蕾;;miR-181a及其靶基因Atg5在胃癌中的表達及臨床意義[J];胃腸病學和肝病學雜志;2016年03期

3 閆兵;任雅麗;何群;席志軍;;自噬相關(guān)蛋白在腎透明細胞癌與癌旁組織中表達狀況的比較研究[J];中華泌尿外科雜志;2013年11期

4 周穎;程勇;吳大保;趙衛(wèi)東;;自噬與腫瘤[J];癌癥進展;2013年02期

5 白海;胡曉燕;馬曉慧;潘耀柱;王存邦;葸瑞;;急性白血病患者骨髓單個核細胞自噬作用的超微結(jié)構(gòu)觀察[J];西北國防醫(yī)學雜志;2012年02期

6 常珍;李春陽;李容林;莫佳莉;;ATG5和ATG7在糜爛型口腔扁平苔蘚中的表達[J];中華口腔醫(yī)學研究雜志(電子版);2011年06期

7 顧龍君;帖利軍;薛惠良;湯靜燕;陳靜;潘慈;陳靜;葉輝;王耀平;董璐;;兒童急性髓系白血病預后因素分析[J];中華血液學雜志;2006年01期

8 ;Molecular mechanism and regulation of autophagy[J];Acta Pharmacologica Sinica;2005年12期

9 謝曉恬,劉振榮,石葦,張虹,劉興元,王耀平;多中心協(xié)作方案序貫治療兒童高危型急性淋巴細胞白血病和晚期淋巴瘤[J];中華兒科雜志;1999年03期

，

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