發(fā)布時(shí)間：2018-01-30 01:03

  本文關(guān)鍵詞： 七氟醚 血栓形成 血管內(nèi)皮細(xì)胞 血小板 凋亡　出處：《延邊大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:研究七氟醚對(duì)大鼠股動(dòng)脈血栓后的影響及其作用機(jī)制。方法:選用體重為250～300g的SD大鼠40只(雌雄各半),隨機(jī)分為4組:對(duì)照組(C組)、模型組(M組)、七氟醚低濃度組(SL組)和七氟醚高濃度組(SH組)。除了對(duì)照組以外,其余3組都用三氯化鐵建立股動(dòng)脈血栓模型,SL組和SH組經(jīng)口持續(xù)吸入濃度分別為3%和4%的七氟醚兩小時(shí),對(duì)照組和模型組則持續(xù)吸入空氣2h。大鼠心尖取血后處死。本實(shí)驗(yàn)測(cè)定大鼠血栓重量;HE染色法觀察大鼠股動(dòng)脈的病理變化;采用ELISA法測(cè)定肝臟,血漿中PF4、vWF、AT-Ⅲ、P-選擇素、BCL-2以及BAX水平;采用蛋白印跡法檢測(cè)大鼠股動(dòng)脈BCL-2和BAX蛋白的表達(dá)水平。結(jié)果:(1)血栓重量稱重結(jié)果:與模型組比較,用藥組的血栓重量都減輕,具有差異性(P0.05或P0.01)。(2)HE染色結(jié)果:對(duì)照組股動(dòng)脈的管腔內(nèi)無(wú)血栓,血管內(nèi)皮完整;模型組股動(dòng)脈管腔內(nèi)可見血栓充盈,血管內(nèi)皮剝脫嚴(yán)重,七氟醚低濃度組血管堵塞較多,血管內(nèi)皮剝脫較重,七氟醚高濃度組血管堵塞較輕,血管內(nèi)皮損傷輕微。(3)ELISA:[1]肝臟指標(biāo)檢測(cè)結(jié)果:1.與對(duì)照組比較,模型組PF4和vWF含量均升高(均P0.05);與模型組比較,用藥組的PF4和vWF含量明顯下降(P0.05)。2.與對(duì)照組比較,模型組P-選擇素和AT-Ⅲ含量均升高(均P0.05);與模型組比較,七氟醚低、高劑量組的P選擇素和AT-Ⅲ含量明顯下降(P0.05,P0.01)。3.和對(duì)照組比較,模型組BAX和BCL-2含量均升高(P0.01);與模型組比較,七氟醚低、高劑量組的BCL-2含量明顯下降(P0.05),而BAX含量明顯下降(P0.01)。[2]血漿指標(biāo)檢測(cè)結(jié)果:1.與對(duì)照組比較,模型組PF4和vWF含量均升高(均P0.05);和模型組比較,七氟醚低、高劑量組的PF4和vWF含量明顯下降(P0.05)。2.與對(duì)照組比較,模型組的BAX明顯升高,BCL-2含量明顯降低(均P0.01);與模型組比較,七氟醚低、高劑量組的BAX含量明顯下降,BCL-2含量明顯升高(均P0.05)。[3]BCL-2和BAX的蛋白表達(dá)水平的變化:與對(duì)照組比較,模型組股動(dòng)脈BAX蛋白表達(dá)量顯著增加;與模型組比較,七氟醚不同濃度組BCL-2明顯升高,BAX明顯下降,(均P0.01),BCL-2/BAX比值明顯升高。結(jié)論:(1)七氟醚對(duì)動(dòng)脈血栓的形成有抑制作用。(2)七氟醚抑制血栓形成的機(jī)制可能是通過保護(hù)血管內(nèi)皮細(xì)胞、防止血小板活化以及抑制細(xì)胞凋亡來(lái)實(shí)現(xiàn)的。
[Abstract]:Objective: to study the effect and mechanism of sevoflurane on femoral artery thrombosis in rats. They were randomly divided into 4 groups: control group (C), model group (M), sevoflurane low concentration group (SL group) and sevoflurane high concentration group (SH group), except for control group. The other three groups were treated with ferric chloride to establish femoral artery thrombosis model. Sevoflurane concentrations of 3% and 4% sevoflurane were continuously inhaled by oral administration in SL and SH groups for two hours, respectively. The rats in the control group and the model group were killed after inhalation of air for 2 hours. The weight of thrombus was measured in this experiment. The pathological changes of femoral artery in rats were observed by HE staining. The levels of P- selectin BCL-2 and BAX in liver and plasma were determined by ELISA. The expression of BCL-2 and BAX protein in femoral artery of rats was detected by Western blot. Results: compared with the model group, the weight of thrombus was reduced in the drug group. The results showed that there was no thrombus in the femoral artery and the vascular endothelium was intact in the control group. In the model group, thrombosis could be seen in femoral artery lumen, vascular endothelium exfoliation was serious, vascular clogging was more in low concentration group of sevoflurane, vascular endothelium exfoliation was more serious, vascular clogging was lighter in high concentration group of sevoflurane. Vascular endothelial injury was mild. [1. Compared with the control group, the contents of PF4 and vWF in the model group were higher than those in the control group (P 0.05). Compared with the model group, the contents of PF4 and vWF in the treatment group decreased significantly (P 0.05N. 2). Compared with the control group, the contents of P- selectin and AT- 鈪，

本文編號(hào)：1474857