發(fā)布時(shí)間：2018-01-31 04:50

  本文關(guān)鍵詞： 癲癇 let-7c 學(xué)習(xí)記憶功能 凋亡　出處：《廣西醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的研究let-7c-1對(duì)戊四氮(pentylenetetrazol,PTZ)致癇大鼠學(xué)習(xí)記憶功能的影響,探討其可能的機(jī)制。方法通過(guò)PTZ腹腔注射SD雄性大鼠建立慢性癲癇模型,隨機(jī)分為癲癇組、干預(yù)對(duì)照組(側(cè)腦室注射let-7c-1-3p agomir control)、let-7c-1激動(dòng)劑組(側(cè)腦室注射let-7c-1-3p agomir),每組12只,另設(shè)12只大鼠為正常對(duì)照組。28天后,觀察各組大鼠的行為學(xué)變化,然后檢測(cè)各組大鼠海馬組織中l(wèi)et-7c-1基因表達(dá)量及Bcl-2蛋白、Caspase3蛋白的表達(dá)。結(jié)果1.行為學(xué)變化結(jié)果顯示:與正常對(duì)照組相比,癲癇組大鼠逃避潛伏期延長(zhǎng)、穿越平臺(tái)次數(shù)減少、在目標(biāo)象限的總路程縮短,差異有統(tǒng)計(jì)學(xué)意義(P0.05);癲癇組與干預(yù)對(duì)照組相比,逃避潛伏期、穿越平臺(tái)次數(shù)、在目標(biāo)象限的總路程無(wú)顯著差異(P0.05);與干預(yù)對(duì)照組相比,let-7c-1激動(dòng)劑組逃避潛伏期明顯延長(zhǎng),穿越平臺(tái)次數(shù)減少、在目標(biāo)象限的總路程縮短,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。2.let-7c-1基因表達(dá):癲癇組let-7c-1基因表達(dá)量高于正常對(duì)照組組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。癲癇組與干預(yù)對(duì)照組相比無(wú)明顯差異(P0.05)。let-7c-1激動(dòng)劑組let-7c-1基因相對(duì)表達(dá)量明顯高于干預(yù)對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。3.Bcl-2、Caspase3蛋白表達(dá):與正常對(duì)照組相比,癲癇組的Bcl-2蛋白表達(dá)減少,Caspase3蛋白表達(dá)增加,差異有統(tǒng)計(jì)學(xué)意義(P0.05);癲癇組與干預(yù)對(duì)照組相比,Bcl-2蛋白及Caspase3蛋白的表達(dá)無(wú)顯著差異(P0.05)。與干預(yù)對(duì)照組相比,let-7c-1激動(dòng)劑組Bcl-2蛋白表達(dá)明顯減少,Caspase3蛋白表達(dá)明顯增加,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論1.let-7c-1可使PTZ致癇大鼠的學(xué)習(xí)記憶功能受損。2.let-7c-1可能通過(guò)減少Bcl-2蛋白表達(dá)及增加Caspase-3蛋白表達(dá)增加神經(jīng)細(xì)胞凋亡而使癲癇大鼠的學(xué)習(xí)記憶功能障礙加重。
[Abstract]:Objective to study the effects of let-7c-1 on learning and memory function in pentylenetetrazolium pentylenetetrazolium pentylenetetrazolium (PTZ) -induced epilepsy rats. Methods male SD rats were injected intraperitoneally with PTZ to establish chronic epilepsy model and were randomly divided into epileptic group. Intervention control group (intraventricular injection of let-7c-1-3p agomir control). Let-7c-1 agonist group (12 rats in each group) were injected with let-7c-1-3p agomirin in lateral ventricle, and 12 rats were used as normal control group for 28 days. The behavioral changes of rats in each group were observed, and then the expression of let-7c-1 gene and Bcl-2 protein in hippocampus of each group were detected. Results 1.Behavioral changes showed that compared with the normal control group, the escape latency of epileptic rats was prolonged, and the times of crossing the platform decreased. 2. The total distance in the target quadrant was shortened, and the difference was statistically significant (P 0.05). Compared with the intervention control group, the Epilepsy group had no significant difference in the total distance in the target quadrant (P 0.05). Compared with the intervention control group, the escape latency of the let-7c-1 agonist group was significantly prolonged, the number of times of crossing the platform was reduced, and the total distance in the target quadrant was shortened. The expression of let-7c-1 gene in epilepsy group was higher than that in normal control group. The difference was statistically significant (P 0.05). There was no significant difference between the epileptic group and the intervention control group (P 0.05). The relative expression of let-7c-1 gene in let-7c-1 agonist group was significantly higher than that in intervention control group. The difference was statistically significant (P 0.05). 3. Expression of Bcl-2Caspase3 protein: compared with normal control group, the expression of Bcl-2 protein in epileptic group was decreased. The expression of Caspase3 protein increased, and the difference was statistically significant (P 0.05). There was no significant difference in the expression of Bcl-2 protein and Caspase3 protein between the epileptic group and the intervention control group, but there was no significant difference between the epileptic group and the intervention control group. In let-7c-1 agonist group, the expression of Bcl-2 protein decreased significantly and the expression of Caspase3 protein increased significantly. The difference was statistically significant (P0.05). Conclusion 1. Let-7 c-1 can impair the learning and memory function of epileptic rats induced by PTZ. 2. Let-7 c-1 may decrease the expression of Bcl-2 protein and increase Caspase-3 eggs. 2. White expression increased neuronal apoptosis and aggravated learning and memory dysfunction in epileptic rats.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R742.1

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