發(fā)布時(shí)間：2018-06-18 02:09

  本文選題：腸球菌 + 氟喹諾酮類藥物��； 參考：《南華大學(xué)》2006年碩士論文

【摘要】：目的：檢測(cè)腸球菌臨床分離株對(duì)3種氟喹諾酮類藥物(FQs)的敏感性，探討腸球菌對(duì)FQs產(chǎn)生耐藥性機(jī)制，研究主動(dòng)外排泵EmeA在腸球菌的分布以及表達(dá)情況，為臨床合理使用抗菌藥物、控制腸球菌感染、研發(fā)新一代抗腸球菌藥物提供理論依據(jù)。 方法：隨時(shí)收集臨床送檢標(biāo)本，按全國(guó)臨床檢驗(yàn)操作規(guī)程腸球菌檢測(cè)方法分離、培養(yǎng)、鑒定和藥敏試驗(yàn)。用二倍瓊脂稀釋法測(cè)定臨床分離株腸球菌對(duì)3種氟喹諾酮類藥物的最低抑菌濃度(MIC)，以及利血平對(duì)MIC值的影響。用聚合酶鏈反應(yīng)(PCR)檢測(cè)編碼主動(dòng)外排泵EmeA的基因emeA在腸球菌臨床株的分布，隨機(jī)挑選一emeA基因陽(yáng)性菌株，將其PCR產(chǎn)物純化后測(cè)序，并對(duì)測(cè)序結(jié)果用Blast軟件進(jìn)行同源性分析，探討腸球菌耐藥與emeA基因的關(guān)系。用逆轉(zhuǎn)錄(RT)—PCR測(cè)定emeA基因陽(yáng)性菌株中emeA mRNA的表達(dá)水平。 結(jié)果：臨床標(biāo)本中分離得到腸球菌共112株，其中糞腸球菌82株，占73.2％；屎腸球菌28株，占25.0％；鳥腸球菌、堅(jiān)韌腸球菌各1株，，各占0.9％。腸球菌主要分離于尿液66株(58.9％)、痰液31株(24.1％)、傷口分泌物10株(11.6％)，而其他標(biāo)本僅有5株(5.4％)。 3種氟喹諾酮類藥物的抗腸球菌活性(MIC_(50)，MIC_(90))從強(qiáng)到弱為：加替沙星分別為1mg／L，4mg／L、司帕沙星為2mg／L，8mg／L和左氧氟沙星為2mg／L，32mg／L。 112株臨床株腸球菌中47株為耐藥菌，占42.0％，其中10株對(duì)3種氟喹諾
[Abstract]:Objective: to detect the sensitivity of clinical isolates of Enterococcus to three fluoroquinolones (FQs), to explore the mechanism of resistance of Enterococcus to FQs, and to study the distribution and expression of EmeA in Enterococcus. It provides theoretical basis for rational use of antimicrobial agents, control of enterococcal infection and development of a new generation of anti-Enterococcal drugs. Methods: clinical specimens were collected at any time, and Enterococcus was isolated, cultured, identified and drug sensitive test according to the national procedure of clinical examination. The minimal inhibitory concentration of Enterococcus to three fluoroquinolones and the effect of reserpine on MIC were determined by double Agar dilution method. The distribution of emeA encoding active efflux pump EmeA gene in clinical strains of Enterococcus was detected by polymerase chain reaction (PCR). A emeA gene positive strain was randomly selected and sequenced after purification. The results were analyzed by blast software. To investigate the relationship between drug resistance of Enterococcus and emeA gene. The expression of emeA in emeA positive strains was detected by reverse transcriptase reverse transcription-polymerase chain reaction (RT-PCR). Results: a total of 112 strains of Enterococcus faecalis (82 strains, 73.2%), 28 strains of Enterococcus faecium (25.0%), 1 strain of Enterococcus faecium and 1 strain of Enterococcus tenacity (0.9%) were isolated from clinical specimens. Enterococcus was mainly isolated from 66 strains of urine (58.9%), sputum from 31 strains of sputum (24. 1%), wound secretions from 10 strains (11. 6%), and other specimens from 5 strains (5. 4%). 3 The anti-Enterococcal activity of fluoroquinolones was as follows: gatifloxacin was 1 mg / L / L 4 mg / L, sparfloxacin was 2 mg / L 8 mg / L and levofloxacin was 2 mg / L 32 mg / L respectively. Of 112 clinical strains of Enterococcus, 47 were drug-resistant bacteria. 42.0, of which 10 were against 3 fluoroquinolones
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R378

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 賈蓓,錢元恕;細(xì)菌主動(dòng)外排系統(tǒng)研究進(jìn)展[J];國(guó)外醫(yī)藥(抗生素分冊(cè));1999年03期

2 饒勇,曾振靈,陳杖榴;抗生素耐藥性的主動(dòng)外排機(jī)制[J];國(guó)外醫(yī)藥(抗生素分冊(cè));2002年03期

3 林東f ,吳n\,張嬰元;腸球菌感染的臨床調(diào)查[J];中國(guó)抗感染化療雜志;2001年02期

4 王清濤,徐英春,王輝,陳民鈞,張秀珍,許淑珍,吳堅(jiān);腸球菌耐藥現(xiàn)狀調(diào)查及抗感染用藥探討[J];中華醫(yī)學(xué)檢驗(yàn)雜志;1999年03期

5 汪復(fù),朱德妹,胡付品,吳n\,張嬰元;上海地區(qū)細(xì)菌耐藥性監(jiān)測(cè)分析[J];中華醫(yī)學(xué)雜志;2001年01期

6 任冰,金巖,謝秀麗,徐英春,陳民鈞;院內(nèi)感染腸球菌耐藥性的觀察[J];中華醫(yī)學(xué)雜志;1997年05期

7 張群智,周惠平;311株腸球菌所致醫(yī)院感染與耐藥性分析[J];中華醫(yī)院感染學(xué)雜志;2000年04期

8 張致平;喹諾酮類抗菌藥研究的新進(jìn)展[J];中國(guó)抗生素雜志;1999年01期

本文編號(hào)：2033534