發(fā)布時間：2018-04-10 01:12

  本文選題：甲狀腺激素　切入點：適量運動　出處：《鄭州大學》2016年碩士論文

【摘要】：背景:甲狀腺激素增高誘發(fā)的甲亢心臟病與適量運動后心肌肥大對心臟收縮泵血、儲備功能的影響常分道揚鑣。前者常伴發(fā)自由基和抗氧化酶的增高,同時心臟的收縮泵血功能、儲備能力增強;而后者常會誘發(fā)心臟的收縮和泵血功能減弱等一系列的反應。2012年Burgoyne報道,在心肌氧應激反應中,雷諾定受體2(Ryanodine Receptor 2,Ry R2)及其相關蛋白可起到重要的介導作用,它是影響心臟收縮泵血能力和儲備功能的重要因素之一。目的:本研究借助所復制的運動和甲亢誘發(fā)的心肌細胞肥大模型,從分子和器官層面探討氧應激反應和Ry R2表達在生理性心肌細胞肥大、病理性心肌細胞肥大過程中的異同,為臨床上甲亢運動療法提供實驗依據。方法:將大鼠隨機的分為六組:對照組、輕度甲狀腺機能亢進組、重度甲狀腺機能亢進組、運動組、輕度甲狀腺機能亢進運動組、重度甲狀腺機能亢進運動組。對照組用0.9%的生理鹽水進行灌胃,輕度甲亢組與重度甲亢組分別行100μg/d與200μg/d左旋甲狀腺素灌胃,運動組則綁負6%的自身重量進行游泳鍛煉,每天鍛煉的時間在30分鐘。經過14天后,測定大鼠甲狀腺的形態(tài)及功能、大鼠心臟的形態(tài)及功能、氧化應激反應、Ry R2的表達。結果:1.甲狀腺形態(tài)和功能:甲亢非運動組大鼠的甲狀腺體積變小,質地變韌,甲狀腺激素FT3經運動后上述變化均有所回復。2.心臟形態(tài)和功能:HE染色顯示,運動組大鼠心肌纖維齊整,膠原纖維齊整、變多;甲亢非運動組大鼠心肌細胞粗、長,亂,有斷裂,出現炎癥細胞;Masson’s顯示,膠原纖維多而亂;超聲心動圖示,甲亢組左心室舒張末期室后壁增厚;ECG示甲亢組R-R間期縮短,HR加快;甲亢運動組大鼠較甲亢非運動組比較,上述變化均有所回復。3.氧應激反應:甲亢組較對照組谷胱甘肽過氧化物酶(Glutathione Peroxidase,GPx)無差異(P0.05),但丙二醛(Malondialdchyde,MDA)含量增高(P0.05);運動組與非運動組比較GPx活力增高和MDA含量均有所增高(P0.05)。4.Ry R2表達:與對照組比較,Ry R2蛋白表達降低(P0.05),甲亢運動組較甲亢非運動組比較,以上的改變發(fā)生了一定程度的回復。結論:借助適量的運動能夠一定范圍內對抗甲狀腺激素所造成的氧應激的病理生理作用。
[Abstract]:Background: the effects of hyperthyroidism induced by thyroid hormone elevation and myocardial hypertrophy after exercise on cardiac systolic pump and reserve function are often diverged.The former is often accompanied by increases in free radicals and antioxidant enzymes, as well as an increase in the systolic pump function and reserve capacity of the heart, while the latter often induces a series of responses, such as the contraction of the heart and the weakening of the pump function, Burgoyne reported in 2012.Renolonidine receptor 2(Ryanodine Receptor 2n Ry R2 and its associated proteins may play an important role in the cardiac oxygen stress response, which is one of the important factors affecting the systolic pump capacity and reserve function of the heart.Objective: to investigate the differences and similarities of oxygen stress response and Ry R2 expression in physiologic and pathological cardiomyocyte hypertrophy by using the model of cardiomyocyte hypertrophy induced by exercise and hyperthyroidism.To provide experimental basis for clinical hyperthyroidism exercise therapy.Methods: the rats were randomly divided into six groups: control group, mild hyperthyroidism group, severe hyperthyroidism group, exercise group, mild hyperthyroidism exercise group and severe hyperthyroidism motor group.The control group was perfused with 0.9% normal saline, the mild hyperthyroidism group and the severe hyperthyroidism group received 100 渭 g / d and 200 渭 g / d L-thyroxine intragastric perfusion respectively, while the exercise group took swimming exercise with negative 6% of their own weight, and the exercise time was 30 minutes per day.After 14 days, the morphology and function of rat thyroid, the shape and function of rat heart and the expression of Ry R2 were measured.The result is 1: 1.Thyroid morphology and function: the thyroid volume and texture of hyperthyroidism rats became smaller and toughened, and the changes of thyroid hormone FT3 recovered to a certain extent after exercise.The cardiac morphology and function of rats showed that myocardial fibers in exercise group were even, collagen fibers were even, and collagen fibers were increased in hyperthyroidism group, but in hyperthyroidism non-exercise group, cardiac myocytes were coarse, long, disorderly, broken, inflammatory cells appeared Massonons showed that collagen fibers were more and more chaotic.Echocardiography showed that the left ventricular end diastolic posterior wall thickening in hyperthyroidism group and ECG showed that R-R interval shortened and HR increased in hyperthyroidism group, and that in hyperthyroidism exercise group compared with hyperthyroidism non-exercise group, the above changes recovered to .3.Oxygen stress response: there was no difference between hyperthyroidism group and control group in glutathione peroxidase (Glutathione peroxidase) (GPX), but the content of malondialdehyde (MDA) in hyperthyroidism group was higher than that in control group (P 0.05). The activity of GPx and the expression of MDA in exercise group were higher than those in non-exercise group (P 0.05). 4. Ry R2 expression in hyperthyroidism group was higher than that in control group.The expression of Ry R2 protein in hyperthyroidism group was lower than that in hyperthyroidism group, and the expression of Ry R2 protein in hyperthyroidism group was lower than that in non-exercise group.The above changes have a certain degree of response.Conclusion: moderate exercise can antagonize the pathophysiological effects of oxygen stress induced by thyroid hormone to a certain extent.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R541.85

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本文編號：1728990