發(fā)布時(shí)間：2018-07-25 13:08

【摘要】：第一章分別介紹了甲基丙烯酸甲酯、雙酚A和硫化氫的衛(wèi)生檢驗(yàn)研究意義和當(dāng)前研究進(jìn)展，并分別簡(jiǎn)要闡述了本課題所建立的甲基丙烯酸甲酯、雙酚A和硫化氫衛(wèi)生檢驗(yàn)新方法的基本原理和定量測(cè)定依據(jù)。 第二章建立了聚甲基丙烯酸甲酯（PMMA）義齒基托材料中殘留甲基丙烯酸甲酯（MMA）的高效液相色譜測(cè)定方法，并分析它在水、生理鹽水、白酒這三種提取液中的溶出特性。提取液經(jīng)Agilent TC-C_(18)色譜柱分離，用乙腈-水（70+30）溶液洗脫，在波長(zhǎng)210nm處進(jìn)行檢測(cè)。MMA濃度為0.05μg/mL～100μg/mL時(shí)，MMA的峰面積與濃度之間呈現(xiàn)良好的線性關(guān)系，檢出限為0.03μg/mL，回收率為99.50%～102.00%。該方法靈敏度高，準(zhǔn)確性好，MMA在52°白酒中的溶出量很大，在生理鹽水中的溶出量較小，在水中的溶出量很小(≤0.44mg/g)。 第三章建立了雙酚A的共振光散射檢測(cè)新方法。在pH8.0的B-R緩沖溶液中，KI04-藏紅T-雙酚A體系發(fā)生氧化還原反應(yīng)和離子締合反應(yīng)，使藏紅T在332nm和560nm處的共振光散射強(qiáng)度都明顯增強(qiáng)，其改變值（ΔI）與雙酚A濃度（ρ）之間呈良好的線性關(guān)系：在332nm處，ρ=0.010～2.40μg/mL時(shí)，線性回歸方程為ΔI＝356.93ρ（μg/mL）+19.77；在560nm處，ρ=0.024～2.40μg/mL時(shí)，ΔI＝245.45ρ（μg/mL）+1.46。檢出限分別為2.86ng/mL和7.10ng/mL。以332nm為測(cè)定波長(zhǎng)時(shí)，樣品加標(biāo)回收率分別為93.88%～103.33%和101.67%～105.00%，相對(duì)標(biāo)準(zhǔn)偏差分別為3.52%～7.80%和4.23%～8.33%。方法簡(jiǎn)單，便宜，靈敏度高。用于塑料薄膜及桶裝飲用水中雙酚A的測(cè)定，結(jié)果滿意。 第四章基于S~(2-)能猝滅熒光素汞的共振熒光，，建立了共振熒光猝滅法測(cè)定生物信使分子硫化氫的新方法。在KH_2PO_4-NaOH緩沖溶液體系中，熒光素汞分子共振熒光強(qiáng)，光譜圖形尖銳，檢測(cè)中熒光干擾因素少。在優(yōu)化的試驗(yàn)條件下，硫化氫濃度在7.38×10~(-8)～1.05×10~(-6)mol/L范圍內(nèi)，體系的ΔF值與硫化氫濃度之間具有良好的線性關(guān)系，線性回歸方程為ΔF=20.62c-5.35（c為硫化氫的濃度，×10~(-7)mol/L），檢出限為2.21×10~(-8)mol/L，加標(biāo)回收率為91.80%～97.45%。該方法靈敏，操作簡(jiǎn)便，用于生物信使分子硫化氫的測(cè)定，結(jié)果滿意。
[Abstract]:In the first chapter, the significance and progress of sanitary test of methyl methacrylate, bisphenol A and hydrogen sulfide are introduced respectively, and the methyl methacrylate which has been established in this subject is briefly described. The basic principle and quantitative determination basis of a new hygienic test method for bisphenol A and hydrogen sulfide. In chapter 2, a high performance liquid chromatography (HPLC) method for the determination of methyl methacrylate (MMA) residue in (PMMA) denture base material was established, and its dissolution characteristics in water, saline and liquor were analyzed. The extract was separated by Agilent TC-C18 column and eluted with acetonitrile-water (7030) solution. The linear relationship between the peak area and concentration of Agilent was observed at the wavelength of 210nm. The detection limit was 0.03 渭 g / mL, and the recovery was 99.50102.00. The method has the advantages of high sensitivity and good accuracy. The dissolution amount of MMA in 52 擄liquor is very large, that in physiological saline is small, and that in water is very small (鈮

本文編號(hào)：2143918