發(fā)布時間：2018-03-08 07:29

  本文選題：線蟲　切入點：批量培養(yǎng)　出處：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：Duddingtonia flagrans是目前發(fā)現(xiàn)的對動物線蟲病生物防治最具有應(yīng)用潛力的一種捕食線蟲性真菌。為了探討其批量培養(yǎng)的最佳方式以及在我國畜牧業(yè)生產(chǎn)實際中的臨床應(yīng)用模式,本研究通過比較D.flagrans的不同培養(yǎng)條件,篩選適用于生產(chǎn)實踐的批量培養(yǎng)基和培養(yǎng)時間。同時,制備D.flagrans凍干生物制劑并進行與常用驅(qū)線蟲藥聯(lián)合使用的試驗,以探討利用捕食線蟲性真菌進行生物防治線蟲感染的臨床應(yīng)用模式,為捕食線蟲性真菌在生產(chǎn)實際中的規(guī)�；瘧�(yīng)用提供基本參考數(shù)據(jù)。研究結(jié)果表明：捕食線蟲性真菌-D.flagrans在培養(yǎng)至第3-4周時,制備成凍干生物制劑,厚壁孢子復(fù)蘇后的萌發(fā)率和菌絲生長率最佳,且玉米粒批量培養(yǎng)基與大麥粒批量培養(yǎng)基統(tǒng)計學(xué)差異不顯著(P0.05),但前者更好一些。凍干生物制劑于4℃冰箱中保存半年以上,孢子的活力沒有發(fā)生明顯變化。捕食線蟲性真菌-D.flagrans凍干生物制劑與伊維菌素(Ivermectin)聯(lián)合應(yīng)用于綿羊的寄生性線蟲病防治,平均幼蟲減少率可達到86.7%；與丙硫咪唑(Albendazole)聯(lián)合應(yīng)用幼蟲減少率更高,為99.2%,而單獨用菌組或單獨用藥組與上述真菌和驅(qū)蟲藥聯(lián)合使用組相比,平均幼蟲減少率差異顯著(P0.05),說明捕食線蟲性真菌-D.flagrans凍干生物制劑與驅(qū)蟲藥聯(lián)合使用的臨床應(yīng)用模式,可以取得較好的寄生性線蟲病臨床防控效果,值得進一步在生產(chǎn)實踐中進行深入研究和推廣。
[Abstract]:Duddingtonia flagrans is one of the most promising nematode predatory fungi for biological control of animal nematode disease. In order to explore the best way of batch culture and clinical application in animal husbandry production in China. In this study, by comparing the different culture conditions of D. flagrans, the batch culture medium and culture time suitable for production practice were selected. Meanwhile, the freeze-dried biological preparations of D. flagrans were prepared and used in combination with common nematode repellent agents. To explore the clinical application model of biological control of nematode infection by nematode predatory fungi. The results showed that the nematode predator fungus -D.flagrans was prepared into freeze-dried biological agent at the 3-4 weeks of culture, and provided the basic reference data for the large-scale application of nematode predatory fungi in the production practice. The germination rate and hyphae growth rate of thick-walled spores were the best after resuscitation, and there was no significant difference between cornkernel batch culture medium and large wheat grain batch medium, but the former was better. Freeze-dried biological agents were preserved in refrigerator at 4 鈩，

本文編號：1582949